|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Human TSPAN1 ORF mammalian expression plasmid, C-GFPSpark tag||HG13073-ACG|
|Human TSPAN1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13073-ACR|
|Human TSPAN1 ORF mammalian expression plasmid, C-Flag tag||HG13073-CF|
|Human TSPAN1 ORF mammalian expression plasmid, C-His tag||HG13073-CH|
|Human TSPAN1 ORF mammalian expression plasmid, C-Myc tag||HG13073-CM|
|Human TSPAN1 ORF mammalian expression plasmid, C-HA tag||HG13073-CY|
|Human TSPAN1 Gene cDNA clone plasmid||HG13073-G|
|Human TSPAN1 ORF mammalian expression plasmid, N-Flag tag||HG13073-NF|
|Human TSPAN1 ORF mammalian expression plasmid, N-His tag||HG13073-NH|
|Human TSPAN1 ORF mammalian expression plasmid, N-Myc tag||HG13073-NM|
|Human TSPAN1 ORF mammalian expression plasmid, N-HA tag||HG13073-NY|
|Human TSPAN1 natural ORF mammalian expression plasmid||HG13073-UT|
|Learn more about expression Vectors|
TSPAN1 belongs to the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins have four hydrophobic domains, intracellular N- and C-termini and two extracellular domains. Tetraspanins act as scaffolding proteins, anchoring multiple proteins to one area of the cell membrane. They also mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. TSPAN1 interacts with human thiamine transporter-1 (hTHTR-1). HTHTR-1 contributes to intestinal thiamine uptake, and its function is regulated at both the transcriptional and posttranscriptional levels. TSPAN1 and hTHTR-1 colocalize in human intestinal epithelial HuTu-80 cells. Coexpression of TSPAN1 in these cells led to a significant decrease in the rate of degradation of hTHTR-1 compared with cells expressing the hTHTR-1 alone; in fact the half-life of the TSPAN1 protein was twice longer in the former cell type compared with the latter cell type.