|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse LGMN / AEP ORF mammalian expression plasmid, C-GFPSpark tag||MG50051-ACG|
|Mouse LGMN / AEP ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50051-ACR|
|Mouse LGMN / AEP ORF mammalian expression plasmid, C-Flag tag||MG50051-CF|
|Mouse LGMN / AEP ORF mammalian expression plasmid, C-His tag||MG50051-CH|
|Mouse LGMN / AEP ORF mammalian expression plasmid, C-Myc tag||MG50051-CM|
|Mouse LGMN / AEP ORF mammalian expression plasmid, C-HA tag||MG50051-CY|
|Mouse LGMN / AEP Gene cDNA clone plasmid||MG50051-M|
|Mouse LGMN / AEP ORF mammalian expression plasmid, N-Flag tag||MG50051-NF|
|Mouse LGMN / AEP ORF mammalian expression plasmid, N-His tag||MG50051-NH|
|Mouse LGMN / AEP ORF mammalian expression plasmid, N-Myc tag||MG50051-NM|
|Mouse LGMN / AEP ORF mammalian expression plasmid, N-HA tag||MG50051-NY|
|Mouse LGMN / AEP natural ORF mammalian expression plasmid||MG50051-UT|
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The Mammalian Legumain, also known as LGMN, also called asparaginyl endopeptidase (AEP), is a cysteine protease belonging to peptidase family C13 with a strict specificity for hydrolysis of asparaginyl bonds. Known previously only from plants and invertebrates, Legumain is discovered as a lysosomal endopeptidase in mammals. Mammalian Legumain is a cysteine endopeptidase, inhibited by iodoacetamide and maleimides, but unaffected by compound E64. The Mammalian Legumain is involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Legumain has been observed to be highly expressed in several types of solid tumors. It was demonstrated in membrane-associated vesicles concentrated at the invadopodia of tumor cells and on cell surfaces where it colocalized with integrins. Legumain was demonstrated to activate progelatinase A. Cells overexpressing Legumain possessed increased migratory and invasive activity in vitro and adopted an invasive and metastatic phenotype in vivo, inferring significance of Legumain in tumor invasion and metastasis. In addition, Legumain is expressed in both murine and human atherosclerotic lesions. The macrophage-specific expression of Legumain in vivo and ability of Legumain to induce chemotaxis of monocytes and endothelial cells in vitro suggest that Legumain may play a functional role in atherogenesis.