|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse CCL20 ORF mammalian expression plasmid, C-GFPSpark tag||MG50048-ACG|
|Mouse CCL20 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50048-ACR|
|Mouse CCL20 ORF mammalian expression plasmid, C-Flag tag||MG50048-CF|
|Mouse CCL20 ORF mammalian expression plasmid, C-His tag||MG50048-CH|
|Mouse CCL20 ORF mammalian expression plasmid, C-Myc tag||MG50048-CM|
|Mouse CCL20 ORF mammalian expression plasmid, C-HA tag||MG50048-CY|
|Mouse CCL20 Gene cDNA clone plasmid||MG50048-M|
|Mouse CCL20 ORF mammalian expression plasmid, N-Flag tag||MG50048-NF|
|Mouse CCL20 ORF mammalian expression plasmid, N-His tag||MG50048-NH|
|Mouse CCL20 ORF mammalian expression plasmid, N-Myc tag||MG50048-NM|
|Mouse CCL20 ORF mammalian expression plasmid, N-HA tag||MG50048-NY|
|Mouse CCL20 natural ORF mammalian expression plasmid||MG50048-UT|
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Chemokine (C-C motif) ligand 20 (CCL20) or liver activation regulated chemokine (LARC) or Macrophage Inflammatory Protein-3 (MIP3A) is a small cytokine belonging to the CC chemokine family that attracts immature dendritic cells and memory T lymphocytes, both expressing CCR6. Depending on the cell type, this chemokine was found to be inducible by cytokines (IL-1beta) and by bacterial, viral, or plant products (including LPS, dsRNA, and PMA). MIP3A / CCL20 is Expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels of MIP3A / CCL20 has been seen in thymus, prostate, testis, small intestine and colon. As a chemotactic factor, MIP3A / CCL20 attracts lymphocytes and, slightly, neutrophils, but not monocytes. This chemokine may Inhibit proliferation of myeloid progenitors in colony formation assays and it may be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. Its C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Chemokine CCL20 was shown to play a role in colorectal cancer (CRC) pathogenesis.