|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse ADAM9 ORF mammalian expression plasmid, C-GFPSpark tag||MG50044-ACG|
|Mouse ADAM9 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50044-ACR|
|Mouse ADAM9 ORF mammalian expression plasmid, C-Flag tag||MG50044-CF|
|Mouse ADAM9 ORF mammalian expression plasmid, C-His tag||MG50044-CH|
|Mouse ADAM9 ORF mammalian expression plasmid, C-Myc tag||MG50044-CM|
|Mouse ADAM9 ORF mammalian expression plasmid, C-HA tag||MG50044-CY|
|Mouse ADAM9 Gene cDNA clone plasmid||MG50044-M|
|Mouse ADAM9 ORF mammalian expression plasmid, N-Flag tag||MG50044-NF|
|Mouse ADAM9 ORF mammalian expression plasmid, N-His tag||MG50044-NH|
|Mouse ADAM9 ORF mammalian expression plasmid, N-Myc tag||MG50044-NM|
|Mouse ADAM9 ORF mammalian expression plasmid, N-HA tag||MG50044-NY|
|Mouse ADAM9 natural ORF mammalian expression plasmid||MG50044-UT|
|Learn more about expression Vectors|
ADAM9 (A disintegrin and metallopeptidase domain 9, MDC9, meltrin gamma), is a type 1 transmembrane protein that has been associated with cancer development and metastases. ADAM9 is consistently overexpressed in various human cancers, and plays a role in tumorigenesis in mouse models. ADAM9 cleaves and releases a number of molecules with important roles in tumorigenesis and angiogenesis, such as EGF, FGFR2iiib, Tie-2, Flk-1, EphB4, CD40, VCAM-1, and VE-cadherin, and could represent a potential therapeutic target in tumors where it is highly expressed. ADAM9 belongs to a family of transmembrane, disintegrin-containing metalloproteinases involved in protein ectodomain shedding and cell-cell and cell-matrix interactions. ADAM-9 adhesive domain plays a role in regulating the motility of cells by interaction with beta1 integrins and modulates MMP synthesis.