|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse bFGF ORF mammalian expression plasmid, C-GFPSpark tag||MG50037-ACG|
|Mouse bFGF ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50037-ACR|
|Mouse bFGF ORF mammalian expression plasmid, N-GFPSpark tag||MG50037-ANG|
|Mouse bFGF ORF mammalian expression plasmid, C-Flag tag||MG50037-CF|
|Mouse bFGF ORF mammalian expression plasmid, C-His tag||MG50037-CH|
|Mouse bFGF ORF mammalian expression plasmid, C-Myc tag||MG50037-CM|
|Mouse bFGF ORF mammalian expression plasmid, C-HA tag||MG50037-CY|
|Mouse bFGF Gene cDNA clone plasmid||MG50037-M|
|Mouse bFGF ORF mammalian expression plasmid, N-Flag tag||MG50037-NF|
|Mouse bFGF ORF mammalian expression plasmid, N-His tag||MG50037-NH|
|Mouse bFGF ORF mammalian expression plasmid, N-Myc tag||MG50037-NM|
|Mouse bFGF ORF mammalian expression plasmid, N-HA tag||MG50037-NY|
|Mouse bFGF natural ORF mammalian expression plasmid||MG50037-UT|
|Learn more about expression Vectors|
Basic fibroblast growth factor (bFGF), also known as FGF2, is a member of the fibroblast growth factor (FGF) family. It is a highly specific chemotactic and mitogenic factor for many cell types, appears to be involved in remodeling damaged tissue, such as ulcer healing, vascular repair, traumatic brain injury (TBI). bFGF is a critical component of human embryonic stem cell culture medium. In addition, bFGF protein is a heparin-binding cationic protein involved in a variety of pathological conditions including angiogenesis and solid tumour growth. Thus, bFGF is regarded as a target for cancers chemopreventive and therapeutic strategies.