|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human CDNF ORF mammalian expression plasmid, C-GFPSpark tag||HG15691-ACG|
|Human CDNF ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG15691-ACR|
|Human CDNF ORF mammalian expression plasmid, C-Flag tag||HG15691-CF|
|Human CDNF ORF mammalian expression plasmid, C-His tag||HG15691-CH|
|Human CDNF ORF mammalian expression plasmid, C-Myc tag||HG15691-CM|
|Human CDNF ORF mammalian expression plasmid, C-HA tag||HG15691-CY|
|Human CDNF Gene cDNA clone plasmid||HG15691-G|
|Human CDNF ORF mammalian expression plasmid, N-Flag tag||HG15691-NF|
|Human CDNF ORF mammalian expression plasmid, N-His tag||HG15691-NH|
|Human CDNF ORF mammalian expression plasmid, N-Myc tag||HG15691-NM|
|Human CDNF ORF mammalian expression plasmid, N-HA tag||HG15691-NY|
|Human CDNF natural ORF mammalian expression plasmid||HG15691-UT|
|Learn more about expression Vectors|
Cerebral Dopamine Neurotrophic Factor (CDNF), also known as ARMETL1 (ARMET-like protein 1), is a secreted protein with eight conserved cysteine residues, predicting a unique protein fold and defining a new, evolutionarily conserved protein family. CDNF is a novel neurotrophic factor with strong trophic activity on dopaminergic neurons comparable to that of glial cell line-derived neurotrophic factor (GDNF). CDNF/ARMETL1 is a evolutionary conserved protein which can protect and restore the function of dopaminergic neurons in the rat model of Parkinson's disease, suggesting that CDNF might be beneficial for the treatment of Parkinson's disease. CDNF is widely expressed in neurons in several brain regions including cerebral cortex, hippocampus, substantia nigra, striatum and cerebellum. Human CDNF is glycosylated and secreted from transiently transfected cells. CDNF promotes the survival, growth, and function of dopamine-specific neurons and is expressed in brain regions that undergo cocaine-induced neuroplasticity.