|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse CTSL / CTSL1 ORF mammalian expression plasmid, C-GFPSpark tag||MG50015-ACG|
|Mouse CTSL / CTSL1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50015-ACR|
|Mouse CTSL / CTSL1 ORF mammalian expression plasmid, C-Flag tag||MG50015-CF|
|Mouse CTSL / CTSL1 ORF mammalian expression plasmid, C-His tag||MG50015-CH|
|Mouse CTSL / CTSL1 ORF mammalian expression plasmid, C-Myc tag||MG50015-CM|
|Mouse CTSL / CTSL1 ORF mammalian expression plasmid, C-HA tag||MG50015-CY|
|Mouse CTSL / CTSL1 ORF mammalian expression plasmid, N-Flag tag||MG50015-NF|
|Mouse CTSL / CTSL1 ORF mammalian expression plasmid, N-His tag||MG50015-NH|
|Mouse CTSL / CTSL1 ORF mammalian expression plasmid, N-Myc tag||MG50015-NM|
|Mouse CTSL / CTSL1 ORF mammalian expression plasmid, N-HA tag||MG50015-NY|
|Mouse CTSL / CTSL1 natural ORF mammalian expression plasmid||MG50015-UT|
|Learn more about expression Vectors|
Cathepsin L is a lysosomal cysteine protease that plays a major role in intracellular protein catabolism, and is potent in degrading collagen, laminin, elastin, as well as alpha-1 protease inhibitor and other structural proteins of basement membranes. It is secreted by liver flukes at all stages of their development in the mammalian host, are believed to play important roles in facilitating parasite migration (tissue degradation), feeding and immuno-evasion. Like many proteases, Cathepsin L is synthesized as an inactive preproenzyme, and cleavage of the 96-residue proregion is necessary to generate the fully active 221-residue mature enzyme. Studies have demonstrated that cleavage of the proregion occur autocatalytically under acidic conditions. The enzyme takes part in nutrient acquisition by catabolizing host proteins to absorbable peptides, facilitates the migration of the parasite through the host intestine and liver by cleaving interstitial matrix proteins such as fibronectin, laminin and native collagen and is implicated in the inactivation of host immune defenses by cleaving immunoglobulins. Recently, Cathepsin L has been shown to suppress Th1 immune response in infected laboratory animals making them susceptible to concurrent bacterial infections. Cathepsin L is synthesized in large amounts and secreted by many malignantly transformed cells, and induced by growth factors and tumor promoters. In addition to its role in protein degradation, evidence has accumulated for the participation of Cathepsin L in various physiological and pathological processes, such as tumor invasion and metastasis, bone resorption, spermatogenesis, and arthritis. Accordingly, Cathepsin L may prove useful as a diagnostic or prognostic marker of human tumor malignancy.