|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Human MICA ORF mammalian expression plasmid, C-GFPSpark tag||HG12302-ACG|
|Human MICA ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG12302-ACR|
|Human MICA ORF mammalian expression plasmid, C-Flag tag||HG12302-CF|
|Human MICA ORF mammalian expression plasmid, C-His tag||HG12302-CH|
|Human MICA ORF mammalian expression plasmid, C-Myc tag||HG12302-CM|
|Human MICA ORF mammalian expression plasmid, C-HA tag||HG12302-CY|
|Human MICA Gene cDNA clone plasmid||HG12302-G|
|Human MICA ORF mammalian expression plasmid, N-Flag tag||HG12302-NF|
|Human MICA ORF mammalian expression plasmid, N-His tag||HG12302-NH|
|Human MICA ORF mammalian expression plasmid, N-Myc tag||HG12302-NM|
|Human MICA ORF mammalian expression plasmid, N-HA tag||HG12302-NY|
|Human MICA natural ORF mammalian expression plasmid||HG12302-UT|
|Learn more about expression Vectors|
MHC class I chain-related molecules A (MICA) is one of the genes in the HLA class I region, which belongs to MHC class I family. It is the member of the non-classical class I family that displays the greatest degree of polymorphism. The MICA protein product is expressed on the cell surface, although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by NK cells, NKT cells, and most of the subtypes of T cells. The Natural killer group 2D (NKG2D), a C-type lectin-like activating immunoreceptor, is a receptor of MICA, which was detected on most gammadelta T cells, CD8+ alphabeta T cells, and natural killer (NK) cells. Effector cells from all these subsets could be stimulated by ligation of NKG2D. Engagement of NKG2D activated cytolytic responses of gammadelta T cells and NK cells against transfectants and epithelial tumor cells expressing MICA. The MICA system is a novel, avidin-free immunohistochemical detection system that provides a significant increase in sensitivity compared to traditional immunodetection systems.