|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse CTSC / DPPI ORF mammalian expression plasmid, C-GFPSpark tag||MG50007-ACG|
|Mouse CTSC / DPPI ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50007-ACR|
|Mouse CTSC / DPPI ORF mammalian expression plasmid, C-Flag tag||MG50007-CF|
|Mouse CTSC / DPPI ORF mammalian expression plasmid, C-His tag||MG50007-CH|
|Mouse CTSC / DPPI ORF mammalian expression plasmid, C-Myc tag||MG50007-CM|
|Mouse CTSC / DPPI ORF mammalian expression plasmid, C-HA tag||MG50007-CY|
|Mouse CTSC / DPPI Gene cDNA clone plasmid||MG50007-M|
|Mouse CTSC / DPPI ORF mammalian expression plasmid, N-Flag tag||MG50007-NF|
|Mouse CTSC / DPPI ORF mammalian expression plasmid, N-His tag||MG50007-NH|
|Mouse CTSC / DPPI ORF mammalian expression plasmid, N-Myc tag||MG50007-NM|
|Mouse CTSC / DPPI ORF mammalian expression plasmid, N-HA tag||MG50007-NY|
|Mouse CTSC / DPPI natural ORF mammalian expression plasmid||MG50007-UT|
|Learn more about expression Vectors|
Cathepsins are proteases found in many types of cells conserved in all animals, which have a vital role in mammalian cellular turnover such as bone resorption. The lysosomal cysteine protease Cathepsin C (CTSC), also known as dipeptidyl peptidase I (DPPI/DPP1), activates a number of granule-associated serine proteases with pro-inflammatory and immune functions by removal of their inhibitory N-terminal dipeptides. This lysosomal exo-cysteine protease belonging to the peptidase C1 family. Active cathepsin C is found in lysosomes as a 200-kDa multimeric enzyme. Subunits constituting this assembly all arise from the proteolytic cleavage of a single precursor giving rise to three peptides: the propeptide, the alpha- and the beta-chains. It is a central coordinator for activation of many serine proteases in immune/inflammatory cells. Defects in the Cathepsin C have been shown to be a cause of Papillon-Lefevre disease, an autosomal recessive disorder characterized by palmoplantar keratosis and periodontitis. Cathepsin C plays a key role in the activation of several degradative enzymes linked to tissue destruction in inflammatory diseases. Thus, it is a therapeutic target for the treatment of a number of inflammatory and autoimmune diseases.