|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Human CAMK1D ORF mammalian expression plasmid, C-GFPSpark tag||HG10728-ACG|
|Human CAMK1D ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10728-ACR|
|Human CAMK1D ORF mammalian expression plasmid, N-GFPSpark tag||HG10728-ANG|
|Human CAMK1D ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG10728-ANR|
|Human CAMK1D ORF mammalian expression plasmid, C-Flag tag||HG10728-CF|
|Human CAMK1D ORF mammalian expression plasmid, C-His tag||HG10728-CH|
|Human CAMK1D ORF mammalian expression plasmid, C-Myc tag||HG10728-CM|
|Human CAMK1D ORF mammalian expression plasmid, C-HA tag||HG10728-CY|
|Human CAMK1D Gene cDNA clone plasmid||HG10728-M|
|Human CAMK1D ORF mammalian expression plasmid, N-Flag tag||HG10728-NF|
|Human CAMK1D ORF mammalian expression plasmid, N-His tag||HG10728-NH|
|Human CAMK1D ORF mammalian expression plasmid, N-Myc tag||HG10728-NM|
|Human CAMK1D ORF mammalian expression plasmid, N-HA tag||HG10728-NY|
|Human CAMK1D natural ORF mammalian expression plasmid||HG10728-UT|
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Calcium/calmodulin-dependent protein kinase or CaM kinases are serine/threonine-specific protein kinases that are primarily regulated by the Calcium/calmodulin complex. These kinases show a memory effect on activation. CaM kinases activity can outlast the intracellular calcium transient that is needed to activate it. In neurons, this property is important for the induction of synaptic plasticity. Pharmacological inhibition of CaM kinases II blocks the induction of long-term potentiation. Upon activation, CaM kinases II phosphorylates postsynaptic glutamate receptors and changes the electrical properties of the synapse.
Calcium/calmodulin-dependent protein kinase type 1D, also known as CaM kinase I delta, CaM kinase ID, CaMKI-like protein kinase, CKLiK and CAMK1D, is a member of the protein kinase superfamily and CaMK subfamily. It contains one protein kinase domain. CAMK1D is broadly expressed. It is highly and mostly expressed in polymorphonuclear leukocytes (neutrophilic and eosinophilic granulocytes) while little or no expression is observed in monocytes and lymphocytes. Engineered overexpression of CAMK1D in non-tumorigenic breast epithelial cells led to increased cell proliferation, and molecular and phenotypic alterations indicative of epithelial-mesenchymal transition (EMT), including loss of cell-cell adhesions and increased cell migration and invasion. CAMK1D is a potential therapeutic target with particular relevance to clinically unfavorable basal-like tumors.