|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse EPOR ORF mammalian expression plasmid, C-GFPSpark tag||MG50031-ACG|
|Mouse EPOR ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50031-ACR|
|Mouse EPOR ORF mammalian expression plasmid, C-Flag tag||MG50031-CF|
|Mouse EPOR ORF mammalian expression plasmid, C-His tag||MG50031-CH|
|Mouse EPOR ORF mammalian expression plasmid, C-Myc tag||MG50031-CM|
|Mouse EPOR ORF mammalian expression plasmid, C-HA tag||MG50031-CY|
|Mouse EPOR Gene cDNA clone plasmid||MG50031-M|
|Mouse EPOR ORF mammalian expression plasmid, N-Flag tag||MG50031-NF|
|Mouse EPOR ORF mammalian expression plasmid, N-His tag||MG50031-NH|
|Mouse EPOR ORF mammalian expression plasmid, N-Myc tag||MG50031-NM|
|Mouse EPOR ORF mammalian expression plasmid, N-HA tag||MG50031-NY|
|Mouse EPOR natural ORF mammalian expression plasmid||MG50031-UT|
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Erythropoietin (EPO) is the major glycoprotein hormone regulator of mammalian erythropoiesis, and is produced by kidney and liver in an oxygen-dependent manner. The biological effects of EPO are mediated by the specific erythropoietin receptor (EPOR/EPO Receptor) on bone marrow erythroblasts, which transmits signals important for both proliferation and differentiation along the erythroid lineage. EPOR protein is a type â… single-transmembrane cytokine receptor, and belongs to the homodimerizing subclass which functions as ligand-induced or ligand-stabilized homodimers. EPOR signaling prevents neuronal death and ischemic injury. Recent studies have shown that EPO and EPOR protein may be involved in carcinogenesis, angiogenesis, and invasion.