|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Human KRAS ORF mammalian expression plasmid, C-GFPSpark tag||HG12259-ACG|
|Human KRAS ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG12259-ACR|
|Human KRAS ORF mammalian expression plasmid, N-GFPSpark tag||HG12259-ANG|
|Human KRAS ORF mammalian expression plasmid, C-Flag tag||HG12259-CF|
|Human KRAS ORF mammalian expression plasmid, C-His tag||HG12259-CH|
|Human KRAS ORF mammalian expression plasmid, C-Myc tag||HG12259-CM|
|Human KRAS ORF mammalian expression plasmid, C-HA tag||HG12259-CY|
|Human KRAS Gene cDNA clone plasmid||HG12259-G|
|Human KRAS ORF mammalian expression plasmid, N-Flag tag||HG12259-NF|
|Human KRAS ORF mammalian expression plasmid, N-His tag||HG12259-NH|
|Human KRAS ORF mammalian expression plasmid, N-Myc tag||HG12259-NM|
|Human KRAS ORF mammalian expression plasmid, N-HA tag||HG12259-NY|
|Human KRAS natural ORF mammalian expression plasmid||HG12259-UT|
|Learn more about expression Vectors|
K-Ras belongs to the small GTPase superfamily, Ras family. As other members of the Ras family, K-Ras is a GTPase and is an early player in many signal transduction pathways. It is usually tethered to cell membranes because of the presence of an isoprenyl group on its C-terminus. K-Ras functions as a molecular on/off switch. Once it is turned on it recruits and activates proteins necessary for the propagation of growth factor and other receptors' signal, such as c-Raf and PI 3-kinase. It binds to GTP in the active state and possesses an intrinsic enzymatic activity which cleaves the terminal phosphate of the nucleotide converting it to GDP. Upon conversion of GTP to GDP, K-Ras is turned off. The rate of conversion is usually slow but can be sped up dramatically by an accessory protein of the GTPase activating protein class, for example RasGAP. In turn K-Ras can bind to proteins of the Guanine Nucleotide Exchange Factor class, for example SOS1, which forces the release of bound nucleotide. Subsequently, K-Ras binds GTP present in the cytosol and the GEF is released from ras-GTP. Besides essential function in normal tissue signaling, the mutation of a K-Ras gene is an essential step in the development of many cancers. Several germline K-Ras mutations have been found to be associated with Noonan syndrome and cardio-facio-cutaneous syndrome. Somatic K-Ras mutations are found at high rates in Leukemias, colon cancer, pancreatic cancer and lung cancer.