|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse ACY1 ORF mammalian expression plasmid, C-GFPSpark tag||MG50008-ACG|
|Mouse ACY1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50008-ACR|
|Mouse ACY1 ORF mammalian expression plasmid, N-GFPSpark tag||MG50008-ANG|
|Mouse ACY1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG50008-ANR|
|Mouse ACY1 ORF mammalian expression plasmid, C-Flag tag||MG50008-CF|
|Mouse ACY1 ORF mammalian expression plasmid, C-His tag||MG50008-CH|
|Mouse ACY1 ORF mammalian expression plasmid, C-Myc tag||MG50008-CM|
|Mouse ACY1 ORF mammalian expression plasmid, C-HA tag||MG50008-CY|
|Mouse ACY1 Gene cDNA clone plasmid||MG50008-M|
|Mouse ACY1 ORF mammalian expression plasmid, N-Flag tag||MG50008-NF|
|Mouse ACY1 ORF mammalian expression plasmid, N-His tag||MG50008-NH|
|Mouse ACY1 ORF mammalian expression plasmid, N-Myc tag||MG50008-NM|
|Mouse ACY1 ORF mammalian expression plasmid, N-HA tag||MG50008-NY|
|Mouse ACY1 natural ORF mammalian expression plasmid||MG50008-UT|
|Learn more about expression Vectors|
Aminoacylase 1 (ACY1), a metalloenzyme that removes amide-linked ACY1 groups from amino acids and may play a role in regulating responses to oxidative stress. Both the C-terminal fragment found in the two-hybrid screen and full-length ACY1 co-immunoprecipitate with SphK1. Though both C-terminal and full-length proteins slightly reduce SphK1 activity measured in vitro, the C-terminal fragment inhibits while full-length ACY1 potentiates the effects of SphK1 on proliferation and apoptosis. It suggested that ACY1 physically interacts with SphK1 and may influence its physiological functions. As a homodimeric zinc-binding enzyme, Aminoacylase 1 catalyzes the hydrolysis of N alpha-acylated amino acids. Deficiency of Aminoacylase 1 due to mutations in the Aminoacylase 1 (ACY1) gene follows an autosomal-recessive trait of inheritance and is characterized by accumulation of N-acetyl amino acids in the urine.