|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse ANPEP ORF mammalian expression plasmid, C-GFPSpark tag||MG50001-ACG|
|Mouse ANPEP ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50001-ACR|
|Mouse ANPEP ORF mammalian expression plasmid, C-Flag tag||MG50001-CF|
|Mouse ANPEP ORF mammalian expression plasmid, C-His tag||MG50001-CH|
|Mouse ANPEP ORF mammalian expression plasmid, C-Myc tag||MG50001-CM|
|Mouse ANPEP ORF mammalian expression plasmid, C-HA tag||MG50001-CY|
|Mouse ANPEP Gene cDNA clone plasmid||MG50001-M|
|Mouse ANPEP ORF mammalian expression plasmid, N-Flag tag||MG50001-NF|
|Mouse ANPEP ORF mammalian expression plasmid, N-His tag||MG50001-NH|
|Mouse ANPEP ORF mammalian expression plasmid, N-Myc tag||MG50001-NM|
|Mouse ANPEP ORF mammalian expression plasmid, N-HA tag||MG50001-NY|
|Mouse ANPEP natural ORF mammalian expression plasmid||MG50001-UT|
|Learn more about expression Vectors|
Aminopeptidase N (ANPEP or APN), also known as CD13, is a cell-surface metalloprotease located in the small-intestinal and renal microvillar membrane, as well as other plasma membranes. It belongs to the peptidase M1 family. CD13 plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases and is involved in the metabolism of regulatory peptides by diverse cell types. CD13/APN is a potent regulator of angiogenesis which is essential for tumor invasion and metastasis, and its transcription in activated endothelial cells is induced by angiogenic growth factors via the RAS/MAPK pathway. In addition, this enzyme has been shown to participate in antigen processing and presentation, and accordingly, defects in this gene appear to be a cause of various types of leukemia or lymphoma and carcinomas.