|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse PCNA ORF mammalian expression plasmid, C-GFPSpark tag||MG51906-ACG|
|Mouse PCNA ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG51906-ACR|
|Mouse PCNA ORF mammalian expression plasmid, N-GFPSpark tag||MG51906-ANG|
|Mouse PCNA ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG51906-ANR|
|Mouse PCNA ORF mammalian expression plasmid, C-Flag tag||MG51906-CF|
|Mouse PCNA ORF mammalian expression plasmid, C-His tag||MG51906-CH|
|Mouse PCNA ORF mammalian expression plasmid, C-Myc tag||MG51906-CM|
|Mouse PCNA ORF mammalian expression plasmid, C-HA tag||MG51906-CY|
|Mouse PCNA Gene cDNA clone plasmid||MG51906-G|
|Mouse PCNA ORF mammalian expression plasmid, N-Flag tag||MG51906-NF|
|Mouse PCNA ORF mammalian expression plasmid, N-His tag||MG51906-NH|
|Mouse PCNA ORF mammalian expression plasmid, N-Myc tag||MG51906-NM|
|Mouse PCNA ORF mammalian expression plasmid, N-HA tag||MG51906-NY|
|Mouse PCNA Gene cDNA clone plasmid||MG51906-U|
|Mouse PCNA natural ORF mammalian expression plasmid||MG51906-UT|
|Learn more about expression Vectors|
Proliferating Cell Nuclear Antigen (PCNA) is a protein only expresse in nomal proliferate cells and cancer cells. It is central to both DNA replication and repair. One of the well-established functions for PCNA is its role as the processivity factor for DNA polymerase delta and epsilon. PCNA tethers the polymerase catalytic unit to the DNA template for rapid and processive DNA synthesis. Two forms of PCNA exist in cells: (i) a detergent-insoluble trimeric form stably associated with the replicating forks during S phase and (ii) a soluble form in quiescent cells in G1 and G2 phases. PCNA forms a toroidal trimer in S phase with replication factor-C (RF-C) and DNA in an ATP-dependent manner and enables the loading of DNA polymerase delta and epsilon onto the complex. The close association of PCNA with kinase complexes involved in cell cycle machinery indicates that PCNA has a regulatory role in cell cycle progression. PCNA also participates in the processing of branched intermediates that arise during the lagging strand DNA synthesis.