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Human PRDX5 Gene cDNA Clone (full-length ORF Clone)

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PRDX5cDNA Clone Product Information
Gene_bank_ref_id:NM_012094.4
cDNA Size:645
cDNA Description:ORF Clone of Homo sapiens peroxiredoxin 5 DNA.
Gene Synonym:PLP, ACR1, B166, PRXV, PMP20, PRDX6, SBBI10, AOEB166, MGC117264, MGC142283, MGC142285, PRDX5
Species:Human
Vector:pGEM-T Vector
Restriction Site:
Tag Sequence:
Sequence Description:Identical with the Gene Bank Ref. ID sequence.
Shipping_carrier:Each tube contains approximately 10 μg of lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
Human PRDX5 Gene cDNA Clone (full-length ORF Clone) on other vectors
Human PRDX5 Gene cDNA Clone (full-length ORF Clone), expression ready, C-GFPSpark-taggedHG11263-ACG$325
Human PRDX5 Gene cDNA Clone (full-length ORF Clone), expression ready, C-OFPSpark tagHG11263-ACR$325
Human PRDX5 Gene cDNA Clone (full-length ORF Clone), expression ready, N-GFPSpark-taggedHG11263-ANG$325
Human PRDX5 Gene cDNA Clone (full-length ORF Clone), expression ready, N-OFPSpark tagHG11263-ANR$325
Human PRDX5 Gene cDNA Clone (full-length ORF Clone), expression ready, C-FLAG-taggedHG11263-CF$295
Human PRDX5 Gene cDNA Clone (full-length ORF Clone), expression ready, C-His-taggedHG11263-CH$295
Human PRDX5 Gene cDNA Clone (full-length ORF Clone), expression ready, C-Myc-taggedHG11263-CM$295
Human PRDX5 Gene cDNA Clone (full-length ORF Clone), expression ready, C-HA-taggedHG11263-CY$295
Human PRDX5 Gene cDNA Clone (full-length ORF Clone)HG11263-G$95
Human PRDX5 Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-taggedHG11263-G-F$295
Human PRDX5 Gene cDNA Clone (full-length ORF Clone), expression ready, untaggedHG11263-G-N$295
Human PRDX5 Gene cDNA Clone (full-length ORF Clone), expression ready, N-FLAG-taggedHG11263-NF$295
Human PRDX5 Gene cDNA Clone (full-length ORF Clone), expression ready, N-His-taggedHG11263-NH$295
Human PRDX5 Gene cDNA Clone (full-length ORF Clone), expression ready, N-Myc-taggedHG11263-NM$295
Human PRDX5 Gene cDNA Clone (full-length ORF Clone), expression ready, N-HA-taggedHG11263-NY$295
Human PRDX5 Gene cDNA Clone (full-length ORF Clone), expression ready, untaggedHG11263-UT$295
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Background

Peroxiredoxin-5, also known as Alu corepressor 1, Antioxidant enzyme B166, Liver tissue 2D-page spot 71B, Peroxisomal antioxidant enzyme, Thioredoxin peroxidase PMP20, Thioredoxin reductase, PRDX5 and ACR1, is cytoplasm protein which belongs to the?peroxiredoxin 2 family. Peroxiredoxin-5 / PRDX5 reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. Peroxiredoxin-5 / PRDX5 is involved in intracellular redox signaling. The Peroxiredoxins / Prx are a family of 25 kDa peroxidases that can reduce H2O2 using an electron from thioredoxin (Trx) or other substances. The mammalian Peroxiredoxins / Prx family is divided into six groups ( PRDX1,PRDX2, PRDX3, PRDX4, PRDX5, PRDX6 ) on the basis of homology of amino acid sequences. They are located in the cytosol and play a role in the cell signaling system. All six mammalian peroxiredoxins are expressed in the lung. Peroxiredoxins / Prx is overexpressed in breast cancer tissues to a great extent suggesting that Peroxiredoxins / Prx has a proliferative effect and may be related to cancer development or progression.

References
  • Seo M.S., et al., 2000, J. Biol. Chem. 275: 20346-54.
  • Declercq J.-P., et al., 2001, J. Mol. Biol. 311:751-9.
  • Noh,D.Y. et al., 2001, Anticancer Res. 21 (3B): 2085-90.
  • Schremmer,B. et al., 2007, Subcell Biochem. 44 :317-44.
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    Catalog:HG11263-G
    List Price: $95.00  (Save $0.00)
    Price:$95.00      [How to order]
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