|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Human BMPR-II ORF mammalian expression plasmid, C-GFPSpark tag||HG10551-ACG|
|Human BMPR-II ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10551-ACR|
|Human BMPR-II ORF mammalian expression plasmid, C-Flag tag||HG10551-CF|
|Human BMPR-II ORF mammalian expression plasmid, C-His tag||HG10551-CH|
|Human BMPR-II ORF mammalian expression plasmid, C-Myc tag||HG10551-CM|
|Human BMPR-II ORF mammalian expression plasmid, C-HA tag||HG10551-CY|
|Human BMPR-II Gene cDNA clone plasmid||HG10551-M|
|Human BMPR-II ORF mammalian expression plasmid, N-Flag tag||HG10551-NF|
|Human BMPR-II ORF mammalian expression plasmid, N-His tag||HG10551-NH|
|Human BMPR-II ORF mammalian expression plasmid, N-Myc tag||HG10551-NM|
|Human BMPR-II ORF mammalian expression plasmid, N-HA tag||HG10551-NY|
|Human BMPR-II natural ORF mammalian expression plasmid||HG10551-UT|
|Learn more about expression Vectors|
The bone morphogenetic protein type II receptor (BMPR-II, or BMPR2), a receptor for the transforming growth factor (TGF)-beta/bone morphogenetic protein (BMP) superfamily. Reduced expression or function of BMPR2 signaling leads to exaggerated TGF-beta signaling and altered cellular responses to TGF-beta. In endothelial cells, BMPR2 mutation increases the susceptibility of cells to apoptosis. BMPR2 transduces BMP signals by forming heteromeric complexes with and phosphorylating BMP type I receptors. The intracellular domain of BMPR2 is both necessary and sufficient for receptor complex interaction. It had been identified that BMPR2 plays a key role in cell growth. Its mutations lead to hereditary pulmonary hypertension, and knockout of Bmpr-II results in early embryonic lethality. The C-terminal tail of BMPR2 provides binding sites for a number of regulatory proteins that may initiate Smad-independent signalling. BMPR2 mutations were predicted to alter the BMP and TGF-b1/SMAD signalling pathways, resulting in proliferation rather than apoptosis of vascular cells, and greatly increase the risk of developing severe pulmonary arterial hypertension. BMPR2 gene result in familial Primary pulmonary hypertension (PPH) transmitted as an autosomal dominant trait, albeit with low penetrance. Heterozygous germline mutations of BMPR2 gene have been identified in patients with familial and sporadic PPH, indicating that BMPR2 may contribute to the maintenance of normal pulmonary vascular structure and function. Tctex-1, a light chain of the motor complex dynein, interacts with the cytoplasmic domain of BMPR2 and demonstrate that Tctex-1 is phosphorylated by BMPR-II, a function disrupted by PPH disease causing mutations within exon 12. BMPR2 and Tctex-1 co-localize to endothelium and smooth muscle within the media of pulmonary arterioles, key sites of vascular remodelling in PPH.