|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse CMBL ORF mammalian expression plasmid, C-GFPSpark tag||MG51405-ACG|
|Mouse CMBL ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG51405-ACR|
|Mouse CMBL ORF mammalian expression plasmid, N-GFPSpark tag||MG51405-ANG|
|Mouse CMBL ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG51405-ANR|
|Mouse CMBL ORF mammalian expression plasmid, C-Flag tag||MG51405-CF|
|Mouse CMBL ORF mammalian expression plasmid, C-His tag||MG51405-CH|
|Mouse CMBL ORF mammalian expression plasmid, C-Myc tag||MG51405-CM|
|Mouse CMBL ORF mammalian expression plasmid, C-HA tag||MG51405-CY|
|Mouse CMBL Gene cDNA clone plasmid||MG51405-G|
|Mouse CMBL ORF mammalian expression plasmid, N-Flag tag||MG51405-NF|
|Mouse CMBL ORF mammalian expression plasmid, N-His tag||MG51405-NH|
|Mouse CMBL ORF mammalian expression plasmid, N-Myc tag||MG51405-NM|
|Mouse CMBL ORF mammalian expression plasmid, N-HA tag||MG51405-NY|
|Mouse CMBL natural ORF mammalian expression plasmid||MG51405-UT|
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Carboxymethylenebutenolidase (CMBL), also known as 4-carboxymethylenebut-2-en-4-olide lactonohydrolase, maleylacetate enol- lactonase, dienelactone hydrolase, and carboxymethylene butenolide hydrolase, is a hydrolase specially belonging to the family of hydrolases. It maily acts on carboxylic ester bonds. CMBL is a human homolog of Pseudomonas dienelactone hydrolase involved in the bacterial halocatechol degradation pathway. The ubiquitous expression of human CMBL gene transcript in various tissues was observed. CMBL was demonstrated to be the primary olmesartan medoxomil (OM) bioactivating enzyme in the liver and intestine. The recombinant human CMBL expressed in mammalian cells was clearly shown to activate OM. The recombinant CMBL also converted other prodrugs having the same ester structure as OM, faropenem medoxomil and lenampicillin, to their active metabolites. CMBL exhibited a unique sensitivity to chemical inhibitors, thus, being distinguishable from other known esterases.