|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human APEX1 ORF mammalian expression plasmid, C-GFPSpark tag||HG16161-ACG|
|Human APEX1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG16161-ACR|
|Human APEX1 ORF mammalian expression plasmid, N-GFPSpark tag||HG16161-ANG|
|Human APEX1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG16161-ANR|
|Human APEX1 ORF mammalian expression plasmid, C-Flag tag||HG16161-CF|
|Human APEX1 ORF mammalian expression plasmid, C-His tag||HG16161-CH|
|Human APEX1 ORF mammalian expression plasmid, C-Myc tag||HG16161-CM|
|Human APEX1 ORF mammalian expression plasmid, C-HA tag||HG16161-CY|
|Human APEX1 Gene cDNA clone plasmid||HG16161-G|
|Human APEX1 ORF mammalian expression plasmid, N-Flag tag||HG16161-NF|
|Human APEX1 ORF mammalian expression plasmid, N-His tag||HG16161-NH|
|Human APEX1 ORF mammalian expression plasmid, N-Myc tag||HG16161-NM|
|Human APEX1 ORF mammalian expression plasmid, N-HA tag||HG16161-NY|
|Human APEX1 natural ORF mammalian expression plasmid||HG16161-UT|
|Learn more about expression Vectors|
The enzyme is known to be a redox factor (Ref-1) stimulating DNA binding activity of AP-1 binding proteins such as Fos and Jun as well as a multifunctional DNA repair enzyme having 5' AP endonuclease, DNA 3' repair diesterase, 3'-5' exonuclease and DNA 3'-phosphatase activities.Although Apex mRNA was expressed ubiquitously, the levels varied significantly, suggesting organ- or tissue-specific expression of the Apex gene. The highest level was observed in the testis, relatively high levels in the thymus, spleen, kidney and brain, and the lowest level in the liver in rats. However, the present results suggested that APEX/Ref-1 gene product can interact with AP-1 binding proteins in brain, especially in the hippocampal formation, to regulate some brain functions by redox-activation.