|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human IGFBP1 ORF mammalian expression plasmid, C-GFPSpark tag||HG16132-ACG|
|Human IGFBP1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG16132-ACR|
|Human IGFBP1 ORF mammalian expression plasmid, C-Flag tag||HG16132-CF|
|Human IGFBP1 ORF mammalian expression plasmid, C-His tag||HG16132-CH|
|Human IGFBP1 ORF mammalian expression plasmid, C-Myc tag||HG16132-CM|
|Human IGFBP1 ORF mammalian expression plasmid, C-HA tag||HG16132-CY|
|Human IGFBP1 Gene cDNA clone plasmid||HG16132-G|
|Human IGFBP1 ORF mammalian expression plasmid, N-Flag tag||HG16132-NF|
|Human IGFBP1 ORF mammalian expression plasmid, N-His tag||HG16132-NH|
|Human IGFBP1 ORF mammalian expression plasmid, N-Myc tag||HG16132-NM|
|Human IGFBP1 ORF mammalian expression plasmid, N-HA tag||HG16132-NY|
|Human IGFBP1 natural ORF mammalian expression plasmid||HG16132-UT|
|Learn more about expression Vectors|
IGFBP1, also known as IGFBP-1 and insulin-like growth factor-binding protein 1, is a member of the insulin-like growth factor binding protein family. IGF binding proteins (IGFBPs) are proteins of 24 to 45 kDa. All six IGFBPs share 50% homology with each other and have binding affinities for IGF-I and IGF-II at the same order of magnitude as the ligands have for the IGF-IR. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. IGFBP1 has an IGFBP domain and a thyroglobulin type-I domain. It binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors.