|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human TNFRSF11A ORF mammalian expression plasmid, C-GFPSpark tag||HG16078-ACG|
|Human TNFRSF11A ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG16078-ACR|
|Human TNFRSF11A ORF mammalian expression plasmid, C-Flag tag||HG16078-CF|
|Human TNFRSF11A ORF mammalian expression plasmid, C-His tag||HG16078-CH|
|Human TNFRSF11A ORF mammalian expression plasmid, C-Myc tag||HG16078-CM|
|Human TNFRSF11A ORF mammalian expression plasmid, C-HA tag||HG16078-CY|
|Human TNFRSF11A Gene cDNA clone plasmid||HG16078-G|
|Human TNFRSF11A ORF mammalian expression plasmid, N-Flag tag||HG16078-NF|
|Human TNFRSF11A ORF mammalian expression plasmid, N-His tag||HG16078-NH|
|Human TNFRSF11A ORF mammalian expression plasmid, N-Myc tag||HG16078-NM|
|Human TNFRSF11A ORF mammalian expression plasmid, N-HA tag||HG16078-NY|
|Human TNFRSF11A natural ORF mammalian expression plasmid||HG16078-UT|
|Learn more about expression Vectors|
TNFRSF11A is a member of the TNF-receptor superfamily. In mouse, it is also known as CD265. TNFRSF11A contains 4 TNFR-Cys repeats and is widely expressed with high levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland. It is an essential mediator for osteoclast and lymph node development. TNFRSF11A and its ligand are important regulators of the interaction between T cells and dendritic cells. It can interact with various TRAF family proteins, through which this receptor induces the activation of NF-kappa B and MAPK8/JNK. Defects in TNFRSF11A can cause familial expansile osteolysis (FEO). FEO is a rare autosomal dominant bone disorder characterized by focal areas of increased bone remodeling. Defects in TNFRSF11A also can cause Paget disease of bone type 2 (PDB2). PDB2 is a bone-remodeling disorder with clinical similarities to FEO. Defects in TNFRSF11A are the cause of osteopetrosis autosomal recessive type 7 which characterized by abnormally dense bone, due to defective resorption of immature bone.