|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Human NEK7 ORF mammalian expression plasmid, C-GFPSpark tag||HG11534-ACG|
|Human NEK7 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11534-ACR|
|Human NEK7 ORF mammalian expression plasmid, N-GFPSpark tag||HG11534-ANG|
|Human NEK7 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG11534-ANR|
|Human NEK7 ORF mammalian expression plasmid, C-Flag tag||HG11534-CF|
|Human NEK7 ORF mammalian expression plasmid, C-His tag||HG11534-CH|
|Human NEK7 ORF mammalian expression plasmid, C-Myc tag||HG11534-CM|
|Human NEK7 ORF mammalian expression plasmid, C-HA tag||HG11534-CY|
|Human NEK7 Gene cDNA clone plasmid||HG11534-M|
|Human NEK7 ORF mammalian expression plasmid, N-Flag tag||HG11534-NF|
|Human NEK7 ORF mammalian expression plasmid, N-His tag||HG11534-NH|
|Human NEK7 ORF mammalian expression plasmid, N-Myc tag||HG11534-NM|
|Human NEK7 ORF mammalian expression plasmid, N-HA tag||HG11534-NY|
|Human NEK7 natural ORF mammalian expression plasmid||HG11534-UT|
|Learn more about expression Vectors|
NIMA (never in mitosis gene a)-related kinase 7, NEK7 belongs to the NIMA subfamily, NEK Ser/Thr protein kinase family, protein kinase superfamily. NEKs (NIMA-related kinases) are mammalian serine/threonine (Ser/Thr) protein kinases structurally related to Aspergillus NIMA (Never in Mitosis, gene A), which plays essential roles in mitotic signaling. NEKs share an amino-terminal catalytic domain related to NIMA, an Aspergillus kinase involved in the control of several aspects of mitosis, and divergent carboxyl-terminal tails of varying length. NEKs are commonly referred to as mitotic kinases, although a definitive in vivo verification of this definition is largely missing. Reduction in the activity of NEK7 or its close paralog, NEK6, has previously been shown to arrest cells in mitosis, mainly at metaphase. NEK7 is a regulator of cell division, and reveal it as an essential component for mammalian growth and survival. The intimate connection between tetraploidy, aneuploidy and cancer development suggests that NEK7 deregulation can induce oncogenesis. The endogenous NEK7 protein is enriched at the centrosome in a microtubule-independent manner. Overexpression of wt or kinase-defective NEK7 resulted in cells of rounder appearance, and higher proportions of multinuclear and apoptotic cells.