|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse CCL3 ORF mammalian expression plasmid, C-GFPSpark tag||MG51114-ACG|
|Mouse CCL3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG51114-ACR|
|Mouse CCL3 ORF mammalian expression plasmid, C-Flag tag||MG51114-CF|
|Mouse CCL3 ORF mammalian expression plasmid, C-His tag||MG51114-CH|
|Mouse CCL3 ORF mammalian expression plasmid, C-Myc tag||MG51114-CM|
|Mouse CCL3 ORF mammalian expression plasmid, C-HA tag||MG51114-CY|
|小鼠 CCL3 基因全长ORF克隆||MG51114-G|
|Mouse CCL3 ORF mammalian expression plasmid, N-Flag tag||MG51114-NF|
|Mouse CCL3 ORF mammalian expression plasmid, N-His tag||MG51114-NH|
|Mouse CCL3 ORF mammalian expression plasmid, N-Myc tag||MG51114-NM|
|Mouse CCL3 ORF mammalian expression plasmid, N-HA tag||MG51114-NY|
|Mouse CCL3 natural ORF mammalian expression plasmid||MG51114-UT|
|Learn more about expression Vectors|
CCL3 is a cytokine belonging to the CC chemokine family. Chemokines are a family of structurally related leukocyte chemoattractant cytokines that play a central role during immunoregulatory and inflammation processes. All chemokines contain four conserved cysteines linked by disulfide bonds, and two major subfamilies, namely CXC and CC, are defined on the basis of the first two cysteines which are separated by one amino acid or are adjacent. CCL3 is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes.