|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human FGFR3 ORF mammalian expression plasmid, C-GFPSpark tag||HG16044-ACG|
|Human FGFR3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG16044-ACR|
|Human FGFR3 ORF mammalian expression plasmid, C-Flag tag||HG16044-CF|
|Human FGFR3 ORF mammalian expression plasmid, C-His tag||HG16044-CH|
|Human FGFR3 ORF mammalian expression plasmid, C-Myc tag||HG16044-CM|
|Human FGFR3 ORF mammalian expression plasmid, C-HA tag||HG16044-CY|
|Human FGFR3 Gene cDNA clone plasmid||HG16044-G|
|Human FGFR3 ORF mammalian expression plasmid, N-Flag tag||HG16044-NF|
|Human FGFR3 ORF mammalian expression plasmid, N-His tag||HG16044-NH|
|Human FGFR3 ORF mammalian expression plasmid, N-Myc tag||HG16044-NM|
|Human FGFR3 ORF mammalian expression plasmid, N-HA tag||HG16044-NY|
|Human FGFR3 natural ORF mammalian expression plasmid||HG16044-UT|
|Learn more about expression Vectors|
FGFR3, also known as CD333, is a member of the fibroblast growth factor receptor (FGFR) family, with its amino acid sequence being highly conserved between members and among divergent species. FGFR family members differ from one another in their ligand affinities and tissue distribution. FGFRs are transmembrane catalytic receptors that have intracellular tyrosine kinase activity. Mutations in FGFR genes are the cause of several human developmental disorders characterized by skeletal abnormalities such as achondroplasia, and upregulation of FGFR expression may lead to cell transformation and cancer. FGFR3, a full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of FGFR3 interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. FGFR3 binds acidic and basic fibroblast growth hormone and plays a role in bone development and maintenance. Mutations in FGFR3 gene lead to craniosynostosis and multiple types of skeletal dysplasia. Three alternatively spliced transcript variants that encode different protein isoforms have been described. CD333 is the receptor for acidic and basic fibroblast growth factors.