|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse IL5 ORF mammalian expression plasmid, C-GFPSpark tag||MG51068-ACG|
|Mouse IL5 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG51068-ACR|
|Mouse IL5 ORF mammalian expression plasmid, C-Flag tag||MG51068-CF|
|Mouse IL5 ORF mammalian expression plasmid, C-His tag||MG51068-CH|
|Mouse IL5 ORF mammalian expression plasmid, C-Myc tag||MG51068-CM|
|Mouse IL5 ORF mammalian expression plasmid, C-HA tag||MG51068-CY|
|Mouse IL5 Gene cDNA clone plasmid||MG51068-G|
|Mouse IL5 ORF mammalian expression plasmid, N-Flag tag||MG51068-NF|
|Mouse IL5 ORF mammalian expression plasmid, N-His tag||MG51068-NH|
|Mouse IL5 ORF mammalian expression plasmid, N-Myc tag||MG51068-NM|
|Mouse IL5 ORF mammalian expression plasmid, N-HA tag||MG51068-NY|
|Mouse IL5 natural ORF mammalian expression plasmid||MG51068-UT|
|Learn more about expression Vectors|
Interleukin 5 (IL-5) is a member of the interleukin family with length of 115 amino acids. Interleukins are a group of cytokines (secreted proteins / signaling molecules) that were first seen to be expressed by white blood cells (leukocytes) and has been found in a wide variety of body cells. Interleukin 5 or IL-5 is produced by T helper-2 cells and mast cells. It helps to stimulate B cell growth and increase immunoglobulin secretion and is considered as a key mediator in eosinophil activation. Interleukin 5 (IL-5) has long been associated with several allergic diseases, including allergic rhinitis and asthma. Growth in the number of circulating, airway tissue, and induced sputum eosinophils have been observed in patients with these diseases. IL-5 also had something with the terminally differentiated granulocyte eosinophils. IL-5 was originally found as an eosinophil colony stimulating factor. It has been proved to be a major regulator of eosinophil accumulation in tissues, and can modulate eosinophil behavior at every stage from maturation to survival.