|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse DPP7 ORF mammalian expression plasmid, C-GFPSpark tag||MG51019-ACG|
|Mouse DPP7 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG51019-ACR|
|Mouse DPP7 ORF mammalian expression plasmid, C-Flag tag||MG51019-CF|
|Mouse DPP7 ORF mammalian expression plasmid, C-His tag||MG51019-CH|
|Mouse DPP7 ORF mammalian expression plasmid, C-Myc tag||MG51019-CM|
|Mouse DPP7 ORF mammalian expression plasmid, C-HA tag||MG51019-CY|
|Mouse DPP7 Gene cDNA clone plasmid||MG51019-G|
|Mouse DPP7 ORF mammalian expression plasmid, N-Flag tag||MG51019-NF|
|Mouse DPP7 ORF mammalian expression plasmid, N-His tag||MG51019-NH|
|Mouse DPP7 ORF mammalian expression plasmid, N-Myc tag||MG51019-NM|
|Mouse DPP7 ORF mammalian expression plasmid, N-HA tag||MG51019-NY|
|Mouse DPP7 natural ORF mammalian expression plasmid||MG51019-UT|
|Learn more about expression Vectors|
DPP7 (dipeptidylpeptidase 7), also known as DPPII and DPP2, is a post-proline cleaving aminopeptidase expressed in quiescent lymphocytes. Dipeptidyl peptidases (DPPs) have post-proline dipeptidyl aminopeptidase activity, cleaving Xaa-Pro dipeptides from the N-termini of proteins. DPPs mediate regulatory activity of their substrates and have been linked to a variety of diseases including type 2 diabetes, obesity and cancer. DPPs can bind specific voltage-gated potassium channels and alter their expression and biophysical properties and may also influence T cells. DPP proteins include DPRP1, DPRP2, DPP3, DPP7, DPP10, DPPX and CD26. It localizes to lysosomes. DPP7 localizes to lysosomes and exists as a homodimer via its leucine zipper motif and is involved in the degradation of oligopeptides. In response to calcium release, it can be secreted in its active form. It is essential for lymphocyte survival, as the inhibition of DPP7 results in quiescent cell apoptosis.