|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse ACP1 ORF mammalian expression plasmid, C-GFPSpark tag||MG51014-ACG|
|Mouse ACP1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG51014-ACR|
|Mouse ACP1 ORF mammalian expression plasmid, N-GFPSpark tag||MG51014-ANG|
|Mouse ACP1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG51014-ANR|
|Mouse ACP1 ORF mammalian expression plasmid, C-Flag tag||MG51014-CF|
|Mouse ACP1 ORF mammalian expression plasmid, C-His tag||MG51014-CH|
|Mouse ACP1 ORF mammalian expression plasmid, C-Myc tag||MG51014-CM|
|Mouse ACP1 ORF mammalian expression plasmid, C-HA tag||MG51014-CY|
|Mouse ACP1 Gene cDNA clone plasmid||MG51014-G|
|Mouse ACP1 ORF mammalian expression plasmid, N-Flag tag||MG51014-NF|
|Mouse ACP1 ORF mammalian expression plasmid, N-His tag||MG51014-NH|
|Mouse ACP1 ORF mammalian expression plasmid, N-Myc tag||MG51014-NM|
|Mouse ACP1 ORF mammalian expression plasmid, N-HA tag||MG51014-NY|
|Mouse ACP1 natural ORF mammalian expression plasmid||MG51014-UT|
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The low molecular weight phosphotyrosine phosphatase (LMW-PTP), also known as Acid phosphatase 1 (ACP1), belongs to the low molecular weight phosphotyrosine protein phosphatase family are involved in the regulation of important physiological functions, including stress resistance and synthesis of the polysaccharide capsule. ACP1/LMW-PTP is an enzyme involved in platelet-derived growth factor-induced mitogenesis and cytoskeleton rearrangement. LMW-PTP is able to specifically bind and dephosphorylate activated PDGF receptor, thus modulating PDGF-induced mitogenesis. In vitro, LMW-PTP was found to efficiently dephosphorylate activated FcgammaRIIA and LAT, but not Syk or phospholipase Cgamma2. The overexpression of LMW-PTP inhibited activation of Syk downstream of FcgammaRIIA and reduced intracellular Ca(2+) mobilization. It been demonstrated that LMW-PTP is responsible for FcgammaRIIA dephosphorylation, and is implicated in the down-regulation of cell activation mediated by this ITAM-bearing immunoreceptor. In addition, ACP1 is a highly polymorphic phosphatase that is especially abundant in the central nervous system and is known to be involved in several signal transduction pathways.