|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse TH ORF mammalian expression plasmid, C-GFPSpark tag||MG50997-ACG|
|Mouse TH ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50997-ACR|
|Mouse TH ORF mammalian expression plasmid, N-GFPSpark tag||MG50997-ANG|
|Mouse TH ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG50997-ANR|
|Mouse TH ORF mammalian expression plasmid, C-Flag tag||MG50997-CF|
|Mouse TH ORF mammalian expression plasmid, C-His tag||MG50997-CH|
|Mouse TH ORF mammalian expression plasmid, C-Myc tag||MG50997-CM|
|Mouse TH ORF mammalian expression plasmid, C-HA tag||MG50997-CY|
|Mouse TH Gene cDNA clone plasmid||MG50997-G|
|Mouse TH ORF mammalian expression plasmid, N-Flag tag||MG50997-NF|
|Mouse TH ORF mammalian expression plasmid, N-His tag||MG50997-NH|
|Mouse TH ORF mammalian expression plasmid, N-Myc tag||MG50997-NM|
|Mouse TH ORF mammalian expression plasmid, N-HA tag||MG50997-NY|
|Mouse TH natural ORF mammalian expression plasmid||MG50997-UT|
|Learn more about expression Vectors|
Tyrosine hydroxylase (TH) is a rate-limiting enzyme in catecholamine synthesis. Tyrosine hydroxylase activity is modulated by protein-protein interactions with enzymes in the same pathway or the tetrahydrobiopterin pathway, structural proteins considered to be chaperones that mediate the neuron's oxidative state. It is phosphorylated at serine (Ser) residues Ser8, Ser19, Ser31 and Ser40 in vitro. The phosphorylation of tyrosine hydroxylase at Ser19 or Ser8 has no direct effect on tyrosine hydroxylase activity. As tyrosine hydroxylase (TH) catalyses the formation of L-DOPA, the rate-limiting step in the biosynthesis of DA, the Parkinson's disease (PD) can be considered as a TH-deficiency syndrome of the striatum. A direct pathogenetic role of TH has also been suggested, as the enzyme is a source of reactive oxygen species (ROS) in vitro and a target for radical-mediated oxidative injury. Recently, it has been demonstrated that L-DOPA is effectively oxidized by mammalian Tyrosine hydroxylase in vitro, possibly contributing to the cytotoxic effects of DOPA.