|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse GPC3 ORF mammalian expression plasmid, C-GFPSpark tag||MG50989-ACG|
|Mouse GPC3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50989-ACR|
|Mouse GPC3 ORF mammalian expression plasmid, C-Flag tag||MG50989-CF|
|Mouse GPC3 ORF mammalian expression plasmid, C-His tag||MG50989-CH|
|Mouse GPC3 ORF mammalian expression plasmid, C-Myc tag||MG50989-CM|
|Mouse GPC3 ORF mammalian expression plasmid, C-HA tag||MG50989-CY|
|Mouse GPC3 Gene cDNA clone plasmid||MG50989-G|
|Mouse GPC3 ORF mammalian expression plasmid, N-Flag tag||MG50989-NF|
|Mouse GPC3 ORF mammalian expression plasmid, N-His tag||MG50989-NH|
|Mouse GPC3 ORF mammalian expression plasmid, N-Myc tag||MG50989-NM|
|Mouse GPC3 ORF mammalian expression plasmid, N-HA tag||MG50989-NY|
|Mouse GPC3 natural ORF mammalian expression plasmid||MG50989-UT|
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Glypican-3, also known as Intestinal protein OCI-5, GPC3, and OCI5, is a member of the glypican family. It belongs to the glypican family and is highly expressed in lung, liver, and kidney. It is a heparan sulfate proteoglycan, which is overexpressed in various neoplasms such as hepatocellular carcinoma, malignant melanoma, and testicular yolk sac tumor, and plays an important role in cell growth and differentiation. GPC3 function is tissue dependent. In some tissues, GPC3 acts as a tumor suppressor gene, whereas in others, it acts as an oncofetal protein. Studies have shown that GPC3 is a reliable marker for hepatocellular carcinoma. The sensitivity and specificity exceeds both alpha-fetoprotein and hepatocyte-paraffin1. GPC3 immunohistochemistry can aid in the differentiation of testicular germ cell tumors, being expressed in all yolk sac tumors but not in seminomas. GPC3 expression has also been identified in some squamous cell carcinomas of the lung and clear cell carcinomas of the ovary. The role of GPC3 in melanomas is still controversial. Thus, Glypican-3 is currently regarded as a tumor marker and potential target for immunotherapy.