|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse DAN / NBL1 ORF mammalian expression plasmid, C-GFPSpark tag||MG50976-ACG|
|Mouse DAN / NBL1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50976-ACR|
|Mouse DAN / NBL1 ORF mammalian expression plasmid, C-Flag tag||MG50976-CF|
|Mouse DAN / NBL1 ORF mammalian expression plasmid, C-His tag||MG50976-CH|
|Mouse DAN / NBL1 ORF mammalian expression plasmid, C-Myc tag||MG50976-CM|
|Mouse DAN / NBL1 ORF mammalian expression plasmid, C-HA tag||MG50976-CY|
|Mouse DAN / NBL1 Gene cDNA clone plasmid||MG50976-G|
|Mouse DAN / NBL1 ORF mammalian expression plasmid, N-Flag tag||MG50976-NF|
|Mouse DAN / NBL1 ORF mammalian expression plasmid, N-His tag||MG50976-NH|
|Mouse DAN / NBL1 ORF mammalian expression plasmid, N-Myc tag||MG50976-NM|
|Mouse DAN / NBL1 ORF mammalian expression plasmid, N-HA tag||MG50976-NY|
|Mouse DAN / NBL1 natural ORF mammalian expression plasmid||MG50976-UT|
|Learn more about expression Vectors|
The Dan (Differential screening-selected gene aberrative in neuroblastoma, also known as N03) gene was first identified as the putative rat tumor suppressor gene and encodes a protein structurally related to Cerberus and Gremlin in vertebrates. It is a founding member of the DAN family of secreted proteins, acts as an inhibitor of cell cycle progression and is closely involved in retinoic acid-induced neuroblastoma differentiation. There are at least five mammalian protein members in the evolutionarily conserved Dan family including DAN, Gremlin/DRM, Cer1 (Cerberus-related), Dante and PRDC (protein related to DAN and cereberus), and share the C-terminal cystine-knot motif. As a secreted glycoprotein, DAN is a member of a class of glycoproteins shown to be secreted inhibitors of the transforming growth factor-beta (TGF-beta) and bone morphogenic protein pathways. It binds to BMPs and preventing their interactions with signaling receptor complexes, and accordingly regulates the processes of embryonic development and tissue differentiation. DAN gene product may have an important role in regulation of the entry of cells into the S phase. In addition, DAN gene product possesses an ability to revert phenotypes of transformed rat fibroblasts and represents a candidate tumour suppressor gene for neuroblastoma.