|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse RPE ORF mammalian expression plasmid, C-GFPSpark tag||MG50968-ACG|
|Mouse RPE ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50968-ACR|
|Mouse RPE ORF mammalian expression plasmid, C-Flag tag||MG50968-CF|
|Mouse RPE ORF mammalian expression plasmid, C-His tag||MG50968-CH|
|Mouse RPE ORF mammalian expression plasmid, C-Myc tag||MG50968-CM|
|Mouse RPE ORF mammalian expression plasmid, C-HA tag||MG50968-CY|
|Mouse RPE Gene cDNA clone plasmid||MG50968-G|
|Mouse RPE ORF mammalian expression plasmid, N-Flag tag||MG50968-NF|
|Mouse RPE ORF mammalian expression plasmid, N-His tag||MG50968-NH|
|Mouse RPE ORF mammalian expression plasmid, N-Myc tag||MG50968-NM|
|Mouse RPE ORF mammalian expression plasmid, N-HA tag||MG50968-NY|
|Mouse RPE natural ORF mammalian expression plasmid||MG50968-UT|
|Learn more about expression Vectors|
The "ribulose phosphate binding" superfamily defined by the Structural Classification of Proteins (SCOP) database is considered the result of divergent evolution from a common (beta/alpha)(8)-barrel ancestor. The superfamily includes d-ribulose 5-phosphate 3-epimerase (RPE), orotidine 5'-monophosphate decarboxylase (OMPDC), and 3-keto-l-gulonate 6-phosphate decarboxylase (KGPDC). Replication of the human genome requires the activation of thousands of replicons distributed along each one of the chromosomes. Each replicon contains an initiation, or origin, site, at which DNA synthesis begins. In enzymology, a L-ribulose-5-phosphate 3-epimerase is an enzyme that catalyzes the chemical reaction L-ribulose 5-phosphate to L-xylulose 5-phosphate. Hence, RPE has one substrate, L-ribulose 5-phosphate, and one product, L-xylulose 5-phosphate. RPE belongs to the family of isomerases, specifically those racemases and epimerases acting on carbohydrates and derivatives. The systematic name of this enzyme class is L-ribulose-5-phosphate 3-epimerase. Other names in common use include L-xylulose 5-phosphate 3-epimerase, UlaE, and SgaU.