|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse GLA ORF mammalian expression plasmid, C-GFPSpark tag||MG50964-ACG|
|Mouse GLA ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50964-ACR|
|Mouse GLA ORF mammalian expression plasmid, C-Flag tag||MG50964-CF|
|Mouse GLA ORF mammalian expression plasmid, C-His tag||MG50964-CH|
|Mouse GLA ORF mammalian expression plasmid, C-Myc tag||MG50964-CM|
|Mouse GLA ORF mammalian expression plasmid, C-HA tag||MG50964-CY|
|Mouse GLA Gene cDNA clone plasmid||MG50964-G|
|Mouse GLA ORF mammalian expression plasmid, N-Flag tag||MG50964-NF|
|Mouse GLA ORF mammalian expression plasmid, N-His tag||MG50964-NH|
|Mouse GLA ORF mammalian expression plasmid, N-Myc tag||MG50964-NM|
|Mouse GLA ORF mammalian expression plasmid, N-HA tag||MG50964-NY|
|Mouse GLA natural ORF mammalian expression plasmid||MG50964-UT|
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Alpha-galactosidase A, also known as Alpha-D-galactoside galactohydrolase, Alpha-D-galactosidase A, Melibiase and GLA, is a member of the glycosyl hydrolase 27 family. GLA is used as a long-term enzyme replacement therapy in patients with a confirmed diagnosis of Fabry disease. Defects in GLA are the cause of Fabry disease (FD) which is a rare X-linked sphingolipidosis disease where glycolipid accumulates in many tissues. The disease consists of an inborn error of glycosphingolipid catabolism. FD patients show systemic accumulation of globotriaoslyceramide (Gb3) and related glycosphingolipids in the plasma and cellular lysosomes throughout the body. Clinical recognition in males results from characteristic skin lesions (angiokeratomas) over the lower trunk. Patients may show ocular deposits, febrile episodes, and burning pain in the extremities. Death results from renal failure, cardiac or cerebral complications of hypertension or other vascular disease. Deficiency of GLA leads to the accumulation of glycosphingolipids in the vasculature leading to multiorgan pathology. In addition to well-described microvascular disease, deficiency of GLA is also characterized by premature macrovascular events such as stroke and possibly myocardial infarction.