|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse CPB2 ORF mammalian expression plasmid, C-GFPSpark tag||MG50963-ACG|
|Mouse CPB2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50963-ACR|
|Mouse CPB2 ORF mammalian expression plasmid, C-Flag tag||MG50963-CF|
|Mouse CPB2 ORF mammalian expression plasmid, C-His tag||MG50963-CH|
|Mouse CPB2 ORF mammalian expression plasmid, C-Myc tag||MG50963-CM|
|Mouse CPB2 ORF mammalian expression plasmid, C-HA tag||MG50963-CY|
|Mouse CPB2 Gene cDNA clone plasmid||MG50963-G|
|Mouse CPB2 ORF mammalian expression plasmid, N-Flag tag||MG50963-NF|
|Mouse CPB2 ORF mammalian expression plasmid, N-His tag||MG50963-NH|
|Mouse CPB2 ORF mammalian expression plasmid, N-Myc tag||MG50963-NM|
|Mouse CPB2 ORF mammalian expression plasmid, N-HA tag||MG50963-NY|
|Mouse CPB2 natural ORF mammalian expression plasmid||MG50963-UT|
|Learn more about expression Vectors|
Carboxypeptidase B2, also known as Carboxypeptidase U, Thrombin-activable fibrinolysis inhibitor, Plasma carboxypeptidase B, CPB2, is a secreted protein which belongs to the peptidase M14 family. Carboxypeptidases are enzymes that hydrolyze C-terminal peptide bonds. The carboxypeptidase family includes metallo-, serine, and cysteine carboxypeptidases. According to their substrate specificity, these enzymes are referred to as carboxypeptidase A (cleaving aliphatic residues) or carboxypeptidase B (cleaving basic amino residues). CPB2 is activated by thrombin and acts on carboxypeptidase B substrates. After thrombin activation, the mature protein downregulates fibrinolysis. CPB2 is synthesized by the liver and circulates in the plasma as a plasminogen-bound zymogen. When it is activated by proteolysis at residue Arg92 by the thrombin / thrombomodulin complex. CPB2 cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. CPB2 exhibits carboxypeptidase activity and activated CPB2 reduces fibrinolysis by removing the fibrin C-terminal residues that are important for the binding and activation of plasminogen.