|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse CNDP1 ORF mammalian expression plasmid, C-GFPSpark tag||MG50959-ACG|
|Mouse CNDP1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50959-ACR|
|Mouse CNDP1 ORF mammalian expression plasmid, N-GFPSpark tag||MG50959-ANG|
|Mouse CNDP1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG50959-ANR|
|Mouse CNDP1 ORF mammalian expression plasmid, C-Flag tag||MG50959-CF|
|Mouse CNDP1 ORF mammalian expression plasmid, C-His tag||MG50959-CH|
|Mouse CNDP1 ORF mammalian expression plasmid, C-Myc tag||MG50959-CM|
|Mouse CNDP1 ORF mammalian expression plasmid, C-HA tag||MG50959-CY|
|Mouse CNDP1 Gene cDNA clone plasmid||MG50959-G|
|Mouse CNDP1 ORF mammalian expression plasmid, N-Flag tag||MG50959-NF|
|Mouse CNDP1 ORF mammalian expression plasmid, N-His tag||MG50959-NH|
|Mouse CNDP1 ORF mammalian expression plasmid, N-Myc tag||MG50959-NM|
|Mouse CNDP1 ORF mammalian expression plasmid, N-HA tag||MG50959-NY|
|Mouse CNDP1 natural ORF mammalian expression plasmid||MG50959-UT|
|Learn more about expression Vectors|
CNDP1, also known as carnosine dipeptidase 1, glutamate carboxypeptidase-like protein 2 (CPGL-2) or carnosinase 1 (CN1), is a member of the M20 metalloprotease family. The CNDP1 gene contains trinucleotide (CTG) repeat length polymorphism in the coding region, which has been demonstrated to be associated with susceptibility to developing diabetic nephropathy, for carnosine protection against the adverse effects of high glucose levels on renal cells. In humans, CNDP1 is secreted from the liver into the serum. In other mammals, including rodents, CNDP1 is expressed exclusively within the kidney and lacks a signal peptide. CNDP1 protein is a secreted homodimeric dipeptidase that specifically hydrolyzes L-carnosine (β-alanyl-L-histidine), and is identified as human carnosinase expressed in the brain. CNDP1 has been associated with diabetic nephropathy in Europeans and European Americans, but not African-Americans. It was identified and confirmed as a risk factor, were cross-sectional and mostly in patients with type 2 diabetes. The polymorphisms of CNDP1 can be excluded as a risk factor for nephropathy in type 1 diabetes. In addition, CNDP1 is also suggested to be implicated in the actions of neuroprotection and neurotransmiting.