|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse VNN1 ORF mammalian expression plasmid, C-GFPSpark tag||MG50952-ACG|
|Mouse VNN1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50952-ACR|
|Mouse VNN1 ORF mammalian expression plasmid, C-Flag tag||MG50952-CF|
|Mouse VNN1 ORF mammalian expression plasmid, C-His tag||MG50952-CH|
|Mouse VNN1 ORF mammalian expression plasmid, C-Myc tag||MG50952-CM|
|Mouse VNN1 ORF mammalian expression plasmid, C-HA tag||MG50952-CY|
|Mouse VNN1 Gene cDNA clone plasmid||MG50952-G|
|Mouse VNN1 ORF mammalian expression plasmid, N-Flag tag||MG50952-NF|
|Mouse VNN1 ORF mammalian expression plasmid, N-His tag||MG50952-NH|
|Mouse VNN1 ORF mammalian expression plasmid, N-Myc tag||MG50952-NM|
|Mouse VNN1 ORF mammalian expression plasmid, N-HA tag||MG50952-NY|
|Mouse VNN1 natural ORF mammalian expression plasmid||MG50952-UT|
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Pantetheinase, also known as Pantetheine hydrolase, Vascular non-inflammatory molecule 1, Vanin-1, and VNN1, is a cell membrane protein which belongs to the CN hydrolase family and BTD/VNN subfamily. Vanin-1 contains one CN hydrolase domain. It is widely expressed with higher expression in spleen, kidney and blood. It is overexpressed in lesional psoriatic skin. Vanin-1 is also a member of the Vanin family of proteins which share extensive sequence similarity with each other, and also with biotinidase. The family includes secreted and membrane-associated proteins, a few of which have been reported to participate in hematopoietic cell trafficking. No biotinidase activity has been demonstrated for any of the vanin proteins, however, they possess pantetheinase activity, which may play a role in oxidative-stress response. Vanin-1 is an epithelial pantetheinase that provides cysteamine to tissue and regulates response to stress. Vanin-1 is expressed by enterocytes, and its absence limits intestinal epithelial cell production of proinflammatory signals. Vanin-1 regulates late adhesion steps of thymus homing under physiological, noninflammatory conditions. The early impact of vanin-1 deficiency on tumor induction was directly correlated to the amount of inflammation and subsequent epithelial proliferation rather than cell death rate. Vanin-1 molecule was shown to be involved in the control of thymus reconstitution following sublethal irradiation.