|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse REG3G ORF mammalian expression plasmid, C-GFPSpark tag||MG50949-ACG|
|Mouse REG3G ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50949-ACR|
|Mouse REG3G ORF mammalian expression plasmid, C-Flag tag||MG50949-CF|
|Mouse REG3G ORF mammalian expression plasmid, C-His tag||MG50949-CH|
|Mouse REG3G ORF mammalian expression plasmid, C-Myc tag||MG50949-CM|
|Mouse REG3G ORF mammalian expression plasmid, C-HA tag||MG50949-CY|
|Mouse REG3G Gene cDNA clone plasmid||MG50949-G|
|Mouse REG3G ORF mammalian expression plasmid, N-Flag tag||MG50949-NF|
|Mouse REG3G ORF mammalian expression plasmid, N-His tag||MG50949-NH|
|Mouse REG3G ORF mammalian expression plasmid, N-Myc tag||MG50949-NM|
|Mouse REG3G ORF mammalian expression plasmid, N-HA tag||MG50949-NY|
|Mouse REG3G natural ORF mammalian expression plasmid||MG50949-UT|
|Learn more about expression Vectors|
Regenerating gene (Reg), first isolated from a regenerating islet cDNA library, encodes a secretory protein with a growth stimulating effect on pancreatic beta cells. Reg and Reg-related genes which were expressed in various organs have been revealed to constitute a multigene family, the Reg family, which consists of four subtypes (types I, II, III, IV) based on the primary structures of the encoded proteins of the genes, which are associated with tissue repair and have been directly implicated in pancreatic beta-cell regeneration. Reg proteins are expressed in various organs and are involved in cancers and neurodegenerative diseases. They display a typical C-type lectin-like domain but possess additional highly conserved amino acids. Regenerating islet-derived 3 gamma (REG3G), also known as pancreatitis-associated protein 1B (PAP1B), is a member of the secreted Reg superfamily and contains one typical C-type lectin domain. REG3G is expressed weakly in pancreas, strongly in intestinal tract, but not in hyperplastic islets REG3G might be a stress protein involved in the control of bacterial proliferation. It was indicated that REG3G specifically targets Gram-positive bacteria because it binds to their surface peptidoglycan layer, and serves as one of several antimicrobial peptides produced by paneth cells via stimulation of toll-like receptors (TLRs) by pathogen-associated molecular patterns (PAMPs).