|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse CLEC4D ORF mammalian expression plasmid, C-GFPSpark tag||MG50946-ACG|
|Mouse CLEC4D ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50946-ACR|
|Mouse CLEC4D ORF mammalian expression plasmid, C-Flag tag||MG50946-CF|
|Mouse CLEC4D ORF mammalian expression plasmid, C-His tag||MG50946-CH|
|Mouse CLEC4D ORF mammalian expression plasmid, C-Myc tag||MG50946-CM|
|Mouse CLEC4D ORF mammalian expression plasmid, C-HA tag||MG50946-CY|
|Mouse CLEC4D Gene cDNA clone plasmid||MG50946-G|
|Mouse CLEC4D ORF mammalian expression plasmid, N-Flag tag||MG50946-NF|
|Mouse CLEC4D ORF mammalian expression plasmid, N-His tag||MG50946-NH|
|Mouse CLEC4D ORF mammalian expression plasmid, N-Myc tag||MG50946-NM|
|Mouse CLEC4D ORF mammalian expression plasmid, N-HA tag||MG50946-NY|
|Mouse CLEC4D natural ORF mammalian expression plasmid||MG50946-UT|
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C-type lectin (CLEC) family is a type of carbohydrate-binding protein domain named lectin. C-type lectins are the most diverse and prevalent lectin family in immunity with its requirement for calcium for binding. Proteins including a C-type lectin domain have diverse range of functions including cell-cell adhesion, immune response to pathogens and apoptosis. There are at least 14 types of C-type lectins: typeⅠto typeⅩⅣ. CLEC4D(CLECSF8) is a typeⅡ membrane glycoprotein belonging to the C-type lectin family, with restricted expression in the monocyte/macrophage lineage. It plays important roles in the function of macrophages.