|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse FCER1A ORF mammalian expression plasmid, C-GFPSpark tag||MG50935-ACG|
|Mouse FCER1A ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50935-ACR|
|Mouse FCER1A ORF mammalian expression plasmid, C-Flag tag||MG50935-CF|
|Mouse FCER1A ORF mammalian expression plasmid, C-His tag||MG50935-CH|
|Mouse FCER1A ORF mammalian expression plasmid, C-Myc tag||MG50935-CM|
|Mouse FCER1A ORF mammalian expression plasmid, C-HA tag||MG50935-CY|
|Mouse FCER1A Gene cDNA clone plasmid||MG50935-G|
|Mouse FCER1A ORF mammalian expression plasmid, N-Flag tag||MG50935-NF|
|Mouse FCER1A ORF mammalian expression plasmid, N-His tag||MG50935-NH|
|Mouse FCER1A ORF mammalian expression plasmid, N-Myc tag||MG50935-NM|
|Mouse FCER1A ORF mammalian expression plasmid, N-HA tag||MG50935-NY|
|Mouse FCER1A natural ORF mammalian expression plasmid||MG50935-UT|
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FcERI, also known as FCER1A, is the alpha subunit of the immunoglobulin epsilon receptor (IgE receptor). IgE receptor is a high affinity IgE receptor which plays a central role in allergic disease, coupling allergen and mast cell to initiate the inflammatory and immediate hypersensitivity responses that are characteristic of disorders such as hay fever and asthma. The allergic response occurs when 2 or more IgE receptors are crosslinked via IgE molecules that in turn are bound to an allergen (antigen) molecule. A perturbation occurs that brings about the release of histamine and proteases from the granules in the cytoplasm of the mast cell and leads to the synthesis of prostaglandins and leukotrienes--potent effectors of the hypersensitivity response. IgE receptor is comprised of an alpha subunit(FcERI), a beta subunit, and two gamma subunits. FcERI is glycosylated and contains 2 Ig-like (immunoglobulin-like) domains.