|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse TRIB3 ORF mammalian expression plasmid, C-GFPSpark tag||MG50888-ACG|
|Mouse TRIB3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50888-ACR|
|Mouse TRIB3 ORF mammalian expression plasmid, N-GFPSpark tag||MG50888-ANG|
|Mouse TRIB3 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG50888-ANR|
|Mouse TRIB3 ORF mammalian expression plasmid, C-Flag tag||MG50888-CF|
|Mouse TRIB3 ORF mammalian expression plasmid, C-His tag||MG50888-CH|
|Mouse TRIB3 ORF mammalian expression plasmid, C-Myc tag||MG50888-CM|
|Mouse TRIB3 ORF mammalian expression plasmid, C-HA tag||MG50888-CY|
|Mouse TRIB3 Gene cDNA clone plasmid||MG50888-G|
|Mouse TRIB3 ORF mammalian expression plasmid, N-Flag tag||MG50888-NF|
|Mouse TRIB3 ORF mammalian expression plasmid, N-His tag||MG50888-NH|
|Mouse TRIB3 ORF mammalian expression plasmid, N-Myc tag||MG50888-NM|
|Mouse TRIB3 ORF mammalian expression plasmid, N-HA tag||MG50888-NY|
|Mouse TRIB3 natural ORF mammalian expression plasmid||MG50888-UT|
|Learn more about expression Vectors|
Tribbles homolog 3, also known as Neuronal cell death-inducible putative kinase, p65-interacting inhibitor of NF-kappa-B, SINK and TRIB3, is a Nucleus protein which belongs to the protein kinase superfamily and CAMK Ser/Thr protein kinase family and Tribbles subfamily. Highest expression Of TRIB3 is in liver, pancreas, peripheral blood leukocytes and bone marrow. It is also highly expressed in a number of primary lung, colon and breast tumors. TRIB3 is expressed in spleen, thymus, and prostate and is undetectable in other examined tissues, including testis, ovary, small intestine, colon, leukocyte, heart, brain, placenta, lung, skeletal muscle, and kidney. TRIB3 disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. TRIB3 may bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. It binds to ATF4 and inhibits its transcriptional activation activity. TRIB3 interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. It interacts with MAPK kinases and regulates activation of MAP kinases. It may play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. TRIB3 does not display kinase activity.