|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human ADH7 ORF mammalian expression plasmid, C-GFPSpark tag||HG15947-ACG|
|Human ADH7 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG15947-ACR|
|Human ADH7 ORF mammalian expression plasmid, N-GFPSpark tag||HG15947-ANG|
|Human ADH7 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG15947-ANR|
|Human ADH7 ORF mammalian expression plasmid, C-Flag tag||HG15947-CF|
|Human ADH7 ORF mammalian expression plasmid, C-His tag||HG15947-CH|
|Human ADH7 ORF mammalian expression plasmid, C-Myc tag||HG15947-CM|
|Human ADH7 ORF mammalian expression plasmid, C-HA tag||HG15947-CY|
|Human ADH7 Gene cDNA clone plasmid||HG15947-G|
|Human ADH7 ORF mammalian expression plasmid, N-Flag tag||HG15947-NF|
|Human ADH7 ORF mammalian expression plasmid, N-His tag||HG15947-NH|
|Human ADH7 ORF mammalian expression plasmid, N-Myc tag||HG15947-NM|
|Human ADH7 ORF mammalian expression plasmid, N-HA tag||HG15947-NY|
|Human ADH7 natural ORF mammalian expression plasmid||HG15947-UT|
|Learn more about expression Vectors|
Calcium/calmodulin-dependent protein kinase type I I subunit alpha, also known as CaM kinase II subunit alpha, CAMKA, and CAMK2A, is a member of the protein kinase superfamily, CAMK Ser/Thr protein kinase family, and CaMK subfamily. CAMK2A contains one protein kinase domain. CAMK2 is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. As a member of the NMDAR signaling complex in excitatory synapses, it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. CAMK2 is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. CAMK2 interacts with BAALC, MPDZ, SYN1, CAMK2N2 and SYNGAP1.