|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse SPOCK1 ORF mammalian expression plasmid, C-GFPSpark tag||MG50832-ACG|
|Mouse SPOCK1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50832-ACR|
|Mouse SPOCK1 ORF mammalian expression plasmid, C-Flag tag||MG50832-CF|
|Mouse SPOCK1 ORF mammalian expression plasmid, C-His tag||MG50832-CH|
|Mouse SPOCK1 ORF mammalian expression plasmid, C-Myc tag||MG50832-CM|
|Mouse SPOCK1 ORF mammalian expression plasmid, C-HA tag||MG50832-CY|
|Mouse SPOCK1 Gene cDNA clone plasmid||MG50832-G|
|Mouse SPOCK1 ORF mammalian expression plasmid, N-Flag tag||MG50832-NF|
|Mouse SPOCK1 ORF mammalian expression plasmid, N-His tag||MG50832-NH|
|Mouse SPOCK1 ORF mammalian expression plasmid, N-Myc tag||MG50832-NM|
|Mouse SPOCK1 ORF mammalian expression plasmid, N-HA tag||MG50832-NY|
|Mouse SPOCK1 natural ORF mammalian expression plasmid||MG50832-UT|
|Learn more about expression Vectors|
Osteonectin, also known as SPOCK1, is an extracellular heparan/chondroitin sulfate proteoglycan. Members of this family are known as testicans, also called SPOCKs. They are characterized structurally by an N-terminal testican-specific domain, a follistatin-like region, a calcium-binding domain, a thyroglobulin-like domain, and an acidic C-terminal domain with two putative glycosaminoglycan attachment sites. SPOCKs are enriched in brain and have been shown to regulate neuronal attachment and outgrowth. They contain inhibitory regions in several domains targeted to different classes of protease, and in some cases may act as protease inhibitors. Osteonectin contains 1 Kazal-like domain and 1 thyroglobulin type-1 domain. Up to now, little is known about osteonectin’s function. It may play a role in cell-cell and cell-matrix interactions. Osteonectin also may contribute to various neuronal mechanisms in the central nervous system.