|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse CDC37 ORF mammalian expression plasmid, C-GFPSpark tag||MG50826-ACG|
|Mouse CDC37 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50826-ACR|
|Mouse CDC37 ORF mammalian expression plasmid, N-GFPSpark tag||MG50826-ANG|
|Mouse CDC37 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG50826-ANR|
|Mouse CDC37 ORF mammalian expression plasmid, C-Flag tag||MG50826-CF|
|Mouse CDC37 ORF mammalian expression plasmid, C-His tag||MG50826-CH|
|Mouse CDC37 ORF mammalian expression plasmid, C-Myc tag||MG50826-CM|
|Mouse CDC37 ORF mammalian expression plasmid, C-HA tag||MG50826-CY|
|Mouse CDC37 Gene cDNA clone plasmid||MG50826-G|
|Mouse CDC37 ORF mammalian expression plasmid, N-Flag tag||MG50826-NF|
|Mouse CDC37 ORF mammalian expression plasmid, N-His tag||MG50826-NH|
|Mouse CDC37 ORF mammalian expression plasmid, N-Myc tag||MG50826-NM|
|Mouse CDC37 ORF mammalian expression plasmid, N-HA tag||MG50826-NY|
|Mouse CDC37 natural ORF mammalian expression plasmid||MG50826-UT|
|Learn more about expression Vectors|
CDC37 is a protein that is expressed in proliferative zones during embryonic development and in adult tissues, consistent with a positive role in proliferation and is required for cell division in budding yeast. CDC37 is though to play an important role in the establishment of signaling pathways controlling cell proliferation through targeting intrinsically unstable oncoprotein kinases such as Cdk-4, Raf-1, and src to the molecular chaperone Hsp90. Decreased Hsp90 expression can reduce the levels of microtubule-associated protein tau, whose overexpression may induce many diseases. CDC37 is considered as a co-chaperone that is classified to Hsp90's accessory proteins. It has been reported that suppression of Cdc37 destabilized tau, leading to its clearance, whereas cdc37 overexpression preserved tau.Cdc37 was found to co-localize with tau in neuronal cells and to physically interact with tau from human brain. Moreover, Cdc37 levels significantly increased with age.