|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse IL1R1 ORF mammalian expression plasmid, C-GFPSpark tag||MG50807-ACG|
|Mouse IL1R1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50807-ACR|
|Mouse IL1R1 ORF mammalian expression plasmid, C-Flag tag||MG50807-CF|
|Mouse IL1R1 ORF mammalian expression plasmid, C-His tag||MG50807-CH|
|Mouse IL1R1 ORF mammalian expression plasmid, C-Myc tag||MG50807-CM|
|Mouse IL1R1 ORF mammalian expression plasmid, C-HA tag||MG50807-CY|
|Mouse IL1R1 Gene cDNA clone plasmid||MG50807-G|
|Mouse IL1R1 ORF mammalian expression plasmid, N-Flag tag||MG50807-NF|
|Mouse IL1R1 ORF mammalian expression plasmid, N-His tag||MG50807-NH|
|Mouse IL1R1 ORF mammalian expression plasmid, N-Myc tag||MG50807-NM|
|Mouse IL1R1 ORF mammalian expression plasmid, N-HA tag||MG50807-NY|
|Mouse IL1R1 natural ORF mammalian expression plasmid||MG50807-UT|
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Interleukin 1 receptor, type I (IL-1R1) also known as CD121a (Cluster of Differentiation 121a), is an interleukin receptor. IL-1R1/CD121a is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein is a receptor for interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I (IL1R1/IL1RA). IL-1R1/CD121a is an important mediator involved in many cytokine induced immune and inflammatory responses. This protein has been characterized by pharmacological and molecular techniques in the mouse brain. The spindle-shaped astrocytes enclose the wound, separating the healthy from damaged neural tissue. The shape change and subsequent repair processes are IL-1β activity-dependent, acting through the IL-1 type 1 receptor (IL-1R1), as co-application of the IL-1type 1 receptor antagonist protein (IL-1ra) blocks IL-1β induced effects. In the spleen, a slight increase in IL-1R AcP and IL-1R1 was observed during the first hours following LPS stimulation. In conclusion, IL-1R AcP mRNA is expressed in the brain and in other tissues where IL-1R1/CD121a transcripts are found. However, the regulation of its expression is distinct from IL-1R1/CD121a. The high level of expression and the lack of regulation of IL-1R AcP transcripts in the brain under inflammatory conditions suggest that the protein might be constitutively expressed in excess.