|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse MCAM ORF mammalian expression plasmid, C-GFPSpark tag||MG50794-ACG|
|Mouse MCAM ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50794-ACR|
|Mouse MCAM ORF mammalian expression plasmid, C-Flag tag||MG50794-CF|
|Mouse MCAM ORF mammalian expression plasmid, C-His tag||MG50794-CH|
|Mouse MCAM ORF mammalian expression plasmid, C-Myc tag||MG50794-CM|
|Mouse MCAM ORF mammalian expression plasmid, C-HA tag||MG50794-CY|
|Mouse MCAM Gene cDNA clone plasmid||MG50794-G|
|Mouse MCAM ORF mammalian expression plasmid, N-Flag tag||MG50794-NF|
|Mouse MCAM ORF mammalian expression plasmid, N-His tag||MG50794-NH|
|Mouse MCAM ORF mammalian expression plasmid, N-Myc tag||MG50794-NM|
|Mouse MCAM ORF mammalian expression plasmid, N-HA tag||MG50794-NY|
|Mouse MCAM natural ORF mammalian expression plasmid||MG50794-UT|
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The CD146 antigen, also known as melanoma cell adhesion molecule (MCAM) and MUC18, is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. CD146 contains the characteristic immunoglobulin-like domains (V-V-C2-C2-C2), a transmembrane region and a short cytoplasmic tail. The CD146 expression is detected in endothelial cells in vascular tissue throughout the body, and plays a role in cell adhesion, as well as in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. As a Ca2+-independent cell adhesion molecule involved in heterophilic cell to cell interactions and a surface receptor, CD146 triggers tyrosine phosphorylation of FYN and PTK2 and subsequently induced signal transduction, proteolysis, or immune recognition. This protein is also expressed predominantly on metastatic lesions and advanced primary tumours, and thus has been suggested to play an important role in tumour progression and the development of metastasis in certain human carcinomas.