|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse CAR3 ORF mammalian expression plasmid, C-GFPSpark tag||MG50780-ACG|
|Mouse CAR3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50780-ACR|
|Mouse CAR3 ORF mammalian expression plasmid, N-GFPSpark tag||MG50780-ANG|
|Mouse CAR3 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG50780-ANR|
|Mouse CAR3 ORF mammalian expression plasmid, C-Flag tag||MG50780-CF|
|Mouse CAR3 ORF mammalian expression plasmid, C-His tag||MG50780-CH|
|Mouse CAR3 ORF mammalian expression plasmid, C-Myc tag||MG50780-CM|
|Mouse CAR3 ORF mammalian expression plasmid, C-HA tag||MG50780-CY|
|Mouse CAR3 Gene cDNA clone plasmid||MG50780-G|
|Mouse CAR3 ORF mammalian expression plasmid, N-Flag tag||MG50780-NF|
|Mouse CAR3 ORF mammalian expression plasmid, N-His tag||MG50780-NH|
|Mouse CAR3 ORF mammalian expression plasmid, N-Myc tag||MG50780-NM|
|Mouse CAR3 ORF mammalian expression plasmid, N-HA tag||MG50780-NY|
|Mouse CAR3 natural ORF mammalian expression plasmid||MG50780-UT|
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Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. Carbonic anhydrases (CAs) form a family of enzymes that catalyze the rapid conversion of carbon dioxide and water to bicarbonate and protons, a reaction that occurs rather slowly in the absence of a catalyst. The active site of most carbonic anhydrases contains a zinc ion, they are therefore classified as metalloenzymes. Several forms of carbonic anhydrase occur in nature. The primary function of the enzyme in animals is to interconvert carbon dioxide and bicarbonate to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide out of tissues. Plants contain a different form called β-carbonic anhydrase, which, from an evolutionary standpoint, is a distinct enzyme, but participates in the same reaction and also uses a zinc ion in its active site.
Carbonic anhydrase 3, also known as Carbonate dehydratase III, CA-III and CA3, is a cytoplasm protein which belongs to the alpha-carbonic anhydrase family. CA3 is activated by proton donors such as imidazole and the dipeptide histidylhistidine. It is inhibited by coumarins and sulfonamide derivatives such as acetazolamide. At 6 weeks gestation, transcripts accumulate at low levels in the somites and at high levels throughout the notochord. As gestation continues, CA3 becomes abundant in all developing muscle masses and continues at high to moderate levels in the notochord.