|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Human CXCL8 ORF mammalian expression plasmid, C-GFPSpark tag||HG10098-ACG|
|Human CXCL8 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10098-ACR|
|Human CXCL8 ORF mammalian expression plasmid, C-Flag tag||HG10098-CF|
|Human CXCL8 ORF mammalian expression plasmid, C-His tag||HG10098-CH|
|Human CXCL8 ORF mammalian expression plasmid, C-Myc tag||HG10098-CM|
|Human CXCL8 ORF mammalian expression plasmid, C-HA tag||HG10098-CY|
|Human CXCL8 Gene cDNA clone plasmid||HG10098-M|
|Human CXCL8 ORF mammalian expression plasmid, N-Flag tag||HG10098-NF|
|Human CXCL8 ORF mammalian expression plasmid, N-His tag||HG10098-NH|
|Human CXCL8 ORF mammalian expression plasmid, N-Myc tag||HG10098-NM|
|Human CXCL8 ORF mammalian expression plasmid, N-HA tag||HG10098-NY|
|Human CXCL8 natural ORF mammalian expression plasmid||HG10098-UT|
|Learn more about expression Vectors|
Interleukin 8 (IL-8), also known as CXCL8, which is a chemokine with a defining CXC amino acid motif that was initially characterized for its leukocyte chemotactic activity, is now known to possess tumorigenic and proangiogenic properties as well. This chemokine is secreted by a variety of cell types including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory stimuli (IL1, TNF, LPS, etc). In human gliomas, IL-8 is expressed and secreted at high levels both in vitro and in vivo, and recent experiments suggest it is critical to glial tumor neovascularity and progression. Levels of IL-8 correlate with histologic grade in glial neoplasms, and the most malignant form, glioblastoma, shows the highest expression in pseudopalisading cells around necrosis, suggesting that hypoxia/anoxia may stimulate expression. Interleukin (IL)-8/CXCL8 is a potent neutrophil chemotactic factor. Accumulating evidence has demonstrated that various types of cells can produce a large amount of IL-8/CXCL8 in response to a wide variety of stimuli, including proinflammatory cytokines, microbes and their products, and environmental changes such as hypoxia, reperfusion, and hyperoxia. Numerous observations have established IL-8/CXCL8 as a key mediator in neutrophil-mediated acute inflammation due to its potent actions on neutrophils. However, several lines of evidence indicate that IL-8/CXCL8 has a wide range of actions on various types of cells, including lymphocytes, monocytes, endothelial cells, and fibroblasts, besides neutrophils. The discovery of these biological functions suggests that IL-8/CXCL8 has crucial roles in various pathological conditions such as chronic inflammation and cancer. IL-8 has been associated with tumor angiogenesis, metastasis, and poor prognosis in breast cancer. IL-8 may present a novel therapeutic target for estrogen driven breast carcinogenesis and tumor progression.