|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse FZD5 ORF mammalian expression plasmid, C-GFPSpark tag||MG50772-ACG|
|Mouse FZD5 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50772-ACR|
|Mouse FZD5 ORF mammalian expression plasmid, C-Flag tag||MG50772-CF|
|Mouse FZD5 ORF mammalian expression plasmid, C-His tag||MG50772-CH|
|Mouse FZD5 ORF mammalian expression plasmid, C-Myc tag||MG50772-CM|
|Mouse FZD5 ORF mammalian expression plasmid, C-HA tag||MG50772-CY|
|Mouse FZD5 Gene cDNA clone plasmid||MG50772-G|
|Mouse FZD5 ORF mammalian expression plasmid, His tag||MG50772-G-H|
|Mouse FZD5 ORF mammalian expression plasmid, N-Flag tag||MG50772-NF|
|Mouse FZD5 ORF mammalian expression plasmid, N-His tag||MG50772-NH|
|Mouse FZD5 ORF mammalian expression plasmid, N-Myc tag||MG50772-NM|
|Mouse FZD5 ORF mammalian expression plasmid, N-HA tag||MG50772-NY|
|Mouse FZD5 natural ORF mammalian expression plasmid||MG50772-UT|
|Learn more about expression Vectors|
Wnt signaling is involved in a variety of embryonic development processes of nonvertebrates and vertebrates, where it determines cell motility, cell polarity, differentiation, proliferation and apoptosis, as well as formation of neural synapses. Various homologs of the Wingless protein, termed WNT factors, represent key mediators and act through a receptor complex comprised of members of the Frizzled and low density lipoprotein-related receptors (LRP). 19 WNTs, 10 Frizzled, and 2 LRP proteins have been identified. Frizzled is a family of G protein-coupled receptor proteins consisting of a divergent signal peptide, a highly conserved extracellular cysteine-rich domain (CRD), a variable-length linker region, a seven-pass transmembrane domain, and a variable-length C-terminal tail.Frizzled 5 (FZD5) is believed to be the receptor for the Wnt5A ligand, and also interactions with Wnt10B, Wnt2B, and Wnt 7A functionally. Recent studies of WNT5A/Frizzled-5 signaling have revealed an unexpected and novel role in orchestrating adaptive immunity in response to microbial stimulation. In addition, FZD5 is also implicated in the survival of mature neurons in the parafascicular nucleus of the thalamus.