|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse CD93 ORF mammalian expression plasmid, C-GFPSpark tag||MG50759-ACG|
|Mouse CD93 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50759-ACR|
|Mouse CD93 ORF mammalian expression plasmid, C-Flag tag||MG50759-CF|
|Mouse CD93 ORF mammalian expression plasmid, C-His tag||MG50759-CH|
|Mouse CD93 ORF mammalian expression plasmid, C-Myc tag||MG50759-CM|
|Mouse CD93 ORF mammalian expression plasmid, C-HA tag||MG50759-CY|
|Mouse CD93 Gene cDNA clone plasmid||MG50759-G|
|Mouse CD93 ORF mammalian expression plasmid, N-Flag tag||MG50759-NF|
|Mouse CD93 ORF mammalian expression plasmid, N-His tag||MG50759-NH|
|Mouse CD93 ORF mammalian expression plasmid, N-Myc tag||MG50759-NM|
|Mouse CD93 ORF mammalian expression plasmid, N-HA tag||MG50759-NY|
|Mouse CD93 natural ORF mammalian expression plasmid||MG50759-UT|
|Learn more about expression Vectors|
CD93 or C1q receptor 1 (C1qR) is an about 120 kDa O-sialoglycoprotein that within the hematopoietic system is selectively expressed on cells of the myeloid lineage. CD93/C1qR is a highly glycosylated transmembrane protein expressed on monocytes, neutrophils, endothelial cells, and stem cells. CD93 was originally identified as a myeloid cell-surface marker and subsequently associated with an ability to modulate phagocytosis of suboptimally opsonized immunoglobulin G and complement particles in vitro. CD93/C1qR, a receptor expressed during early B-cell development, is reinduced during plasma-cell differentiation. High CD93/CD138 expression was restricted to antibody-secreting cells both in T-dependent and T-independent responses as naive, memory, and germinal-center B cells remained CD93-negative. CD93 was expressed on (pre)plasmablasts/plasma cells, including long-lived plasma cells that showed decreased cell cycle activity, high levels of isotype-switched Ig secretion, and modification of the transcriptional network. CD93 is important for the maintenance of plasma cells in bone marrow niches.