|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Human FAM3B ORF mammalian expression plasmid, C-GFPSpark tag||HG11338-ACG|
|Human FAM3B ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11338-ACR|
|Human FAM3B ORF mammalian expression plasmid, C-Flag tag||HG11338-CF|
|Human FAM3B ORF mammalian expression plasmid, C-His tag||HG11338-CH|
|Human FAM3B ORF mammalian expression plasmid, C-Myc tag||HG11338-CM|
|Human FAM3B ORF mammalian expression plasmid, C-HA tag||HG11338-CY|
|Human FAM3B Gene cDNA clone plasmid||HG11338-M|
|Human FAM3B ORF mammalian expression plasmid, N-Flag tag||HG11338-NF|
|Human FAM3B ORF mammalian expression plasmid, N-His tag||HG11338-NH|
|Human FAM3B ORF mammalian expression plasmid, N-Myc tag||HG11338-NM|
|Human FAM3B ORF mammalian expression plasmid, N-HA tag||HG11338-NY|
|Human FAM3B natural ORF mammalian expression plasmid||HG11338-UT|
|Learn more about expression Vectors|
Pancreatic derived factor, also known as FAM3B, is an islet-specific secreted cytokine specifically expressed at high levels in the islets of Langerhans of the endocrine pancreas. FAM3B protein is present in alpha- and beta- cells of pancreatic islets, insulin-secreting beta-TC3 cells, and glucagon-secreting alpha-TC cells. FAM3B causes apoptosis of beta-cells as assessed by electron microscopy, annexin Ⅴ fluorescent staining, and flow-cytometric terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling assay. FAM3B activated caspase-3 while not affect cytosolic Ca2+ levels or nitric oxide levels. Hense, FAM3B may have a role in the process of pancreatic?-cell apoptosis of primary islet and cell lines. FAM3B secretion is regulated by glucose and other insulin secretagogues. This islet-specific secreted cytokine is secreted from both pancreatic alpha- and beta- cells. Glucose stimulates FAM3B secretion dose dependently in beta- cell lines and primary islets but not in alpha-cells. It is likely cosecreted with insulin via the same regulatory mechanisms and structure and conformation is vital for FAM3B secretion.