|Datasheet||Specific References||Reviews||Related Products||Protocols|
|A DNA sequence encoding the rat TNFSF12 (Arg105-His249) was expressed with Fc region of human IgG1 at the N-terminus.|
|In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.|
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
|> 85 % as determined by SDS-PAGE|
|1. Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 for this effect is typically 5-20 ng/mL.|
2. Immobilized Fc-rat TNFSF12 at 10 μg/ml (100 μl/well) can bind biotinylated human Fc-TNFRSF12A (Cat:10431-H01H), The EC50 of biotinylated human Fc-TNFRSF12A (Cat:10431-H01H) is 0.12-0.28 μg/ml.
|< 1.0 EU per μg of the protein as determined by the LAL method|
|Samples are stable for up to twelve months from date of receipt at -70℃|
|The recombinant rat TNFSF12 comprises 405 amino acids and predicts a molecular mass of 44.4 kDa. The apparent molecular mass of the recombinant protein is approximately 49 kDa in SDS-PAGE under reducing conditions due to glycosylation.|
|Lyophilized from sterile PBS, pH 7.4|
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
|Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.|
|A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.|
TNFSF12 is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. It is a ligand for the FN14/TWEAKR receptor. TNFSF12 has overlapping signaling functions with TNF, but displays a much wider tissue distribution. It can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. It is also found that TNFSF12 promotes proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis. TNFSF12 also is a weak inducer of apoptosis in some cell types and mediates NF-kappa-B activation.